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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD135 / FLT3 Antibody (clone A2F10) LS‑C106578
FLT3 antibody LS-C106578 is an unconjugated rat monoclonal antibody to mouse FLT3 (CD135). Validated for Flow and IP.
Catalog
Size
Price
LS-C106578-50
50 µg (0.5 mg/ml)
Unavailable
LS-C106578-100
100 µg (0.5 mg/ml)
Unavailable
LS-C106578-500
500 µg (0.5 mg/ml)
Unavailable

Popular CD135 / FLT3 Products

Anti-FLT3 / CD135 antibody LS-A7147 IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Anti-FLT3 / CD135 antibody LS-A7148 IHC of human interfollicular zone. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Species: Human, Mouse
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Anti-FLT3 / CD135 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4049 concentration 20 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Flow Cytometry

Product Description

FLT3 antibody LS-C106578 is an unconjugated rat monoclonal antibody to mouse FLT3 (CD135). Validated for Flow and IP.
About CD135 / FLT3
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. P36888 NM_004119 NP_004110.2

FLT3 Antibody, CD135 Antibody, CD135 antigen Antibody, Fetal liver kinase 2 Antibody, Fetal liver kinase-2 Antibody, FL cytokine receptor Antibody, FLK2 Antibody, FLT-3 Antibody, Fms-like tyrosine kinase 3 Antibody, Stem cell tyrosine kinase 1 Antibody, STK-1 Antibody, FLK-2 Antibody, Fms-related tyrosine kinase 3 Antibody, STK1 Antibody


Specifications

Target
Mouse CD135 / FLT3
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [A2F10]
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunoprecipitation
  • Flow Cytometry
Immunogen
CD135 / FLT3 antibody was raised against mouse FLT3
Usage
The A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Flow Cytometry

Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.

Flow Cytometry

Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.

Flow Cytometry

Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.

Flow Cytometry

Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.


Requested From: 
Date Requested: 6/25/2018

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