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CD135 / FLT3 Antibody (aa33‑64) LS‑C97535

Catalog Number Size Price
LS-C97535-400 400 µl $325 
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-FLT3 antibody in HL-60 cell lysate. FLT3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
FLT3 (CD135) Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-FLT3 antibody in HL-60 cell lysate. FLT3 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
FLT3 (CD135) Antibody flow cytometry of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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CD135 / FLT3 Antibody (aa33‑64) LS‑C97535

Antibody:
Rabbit Polyclonal to Human CD135 / FLT3
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$325 
LS-C97535-400
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CD135 / FLT3
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FLT3 antibody LS-C97535 is an unconjugated rabbit polyclonal antibody to human FLT3 (CD135 ). Validated for Flow, IHC and WB.
Target
Human CD135 / FLT3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E9145 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97535. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa33-64
Specificity
This FLT3 (CD135) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-64 amino acids from the N-terminal region of human FLT3 (CD135).
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD135 / FLT3
P36888 NM_004119 NP_004110.2

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Two-color surface staining of mouse bone marrow PE anti-mouse CD135 (A2F10) (right) and FITC CD45R/B220 (RA3-6B2). Total viable cells were used for analysis.
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Requested From: United States
Date Requested: 9/22/2019