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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCL5 / RANTES Antibody (clone 8.PP.11) IHC‑plus™ LS‑B2472 Best Seller
Note: This antibody replaces LS-C17596
RANTES antibody LS-B2472 is an unconjugated mouse monoclonal antibody to human RANTES (CCL5). Validated for ELISA, IHC and Neut. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2472-50
50 µg (1 mg/ml)
Unavailable

Popular CCL5 / RANTES Products

Western Blot (reducing) of CCL5 / RANTES antibody LS-C104689.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Western Blot (reducing) of CCL5 / RANTES antibody LS-C104758.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

RANTES antibody LS-B2472 is an unconjugated mouse monoclonal antibody to human RANTES (CCL5). Validated for ELISA, IHC and Neut. Tested on 20 paraffin-embedded human tissues.
About CCL5 / RANTES
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). P13501 NM_002985 NP_002976.2

CCL5 Antibody, Chemokine (C-C motif) ligand 5 Antibody, D17S136E Antibody, EoCP Antibody, T-cell specific protein p288 Antibody, SCYA5 Antibody, SISd Antibody, T cell-specific protein P228 Antibody, Beta-chemokine RANTES Antibody, C-C motif chemokine 5 Antibody, RANTES Antibody, SIS-delta Antibody, Small-inducible cytokine A5 Antibody, T-cell-specific protein RANTES Antibody, TCP228 Antibody


Specifications

Target
Human CCL5 / RANTES
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [8.PP.11]
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ELISA (2 - 4 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CCL5 / RANTES antibody was raised against rANTES.
Specificity
Recognizes human RANTES.
Usage
Immunohistochemistry: LS-B2472 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2472 was determined to be 5 ug/ml. Neutralization: 5-7 ug/ml to yield maximum inhibition (ND50) of hRANTES at 100 ng/ml).
Presentation
PBS
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.
Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.

Immunohistochemistry

Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.
Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.

Immunohistochemistry

Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.
Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.

Immunohistochemistry

Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.
Anti-CCL5 / RANTES antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2472 concentration 5 ug/ml.


Requested From: 
Date Requested: 6/20/2018

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