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Catalog Number Size Price
LS-C104450-500 500 µg $326 
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Western Blot Reduced
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Western Blot Unreduced
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Sandwich ELISA
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Western Blot Reduced
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Western Blot Unreduced
CCL5 / RANTES Antibody - Anti-Human RANTES (CCL5) Sandwich ELISA
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Monoclonal Mouse anti‑Human CCL5 / RANTES Antibody (WB) LS‑C104450

Monoclonal Mouse anti‑Human CCL5 / RANTES Antibody (WB) LS‑C104450

Note: This antibody replaces LS-C54350
Antibody:
CCL5 / RANTES Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$326
LS-C104450-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCL5 / RANTES Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RANTES antibody LS-C104450 is an unconjugated mouse monoclonal antibody to human RANTES (CCL5). Validated for ELISA and WB.
Target
Human CCL5 / RANTES
Synonyms
CCL5 | Chemokine (C-C motif) ligand 5 | D17S136E | EoCP | T-cell specific protein p288 | SCYA5 | SISd | T cell-specific protein P228 | Beta-chemokine RANTES | C-C motif chemokine 5 | RANTES | SIS-delta | Small-inducible cytokine A5 | T-cell-specific protein RANTES | TCP228
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Human RANTES.
Specificity
Human RANTES
Applications
  • Western blot (0.5 - 1 µg/ml)
  • ELISA (2 - 4 µg/ml)
Usage
Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3-5 ug/ml of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 ul/well), a concentration of 2-4 ug/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 ug/ml. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL5 / RANTES
P13501 NM_002985 NP_002976.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/28/2024