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Anti-CCL2 / MCP1 Antibody LS-C154086

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl 1 mg/ml $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL2 / MCP1 Antibodies

Anti-CCL2 / MCP1 Antibody (aa102-130, clone ECE.2) LS-C139852
Rat Monoclonal [clone ECE.2] (IgG1) to Mouse CCL2 / MCP1
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-CCL2 / MCP1 Antibody (clone 2D8) LS-C169178
Mouse Monoclonal [clone 2D8] (IgG1) to Human CCL2 / MCP1
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human CCL2 / MCP1
Human
Western blot, ELISA
Unconjugated

Details

Human CCL2 / MCP1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

CCL2 / MCP1 antibody was raised against this IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E. coli.
This antibody is specific for human MCP-1 protein. A BLAST analysis was used to suggest cross-reactivity with MCP-1 from human sources based on 100% homology with the immunizing sequence. Based on 98 to 100% homology, there is a chance of cross-reactivity to MCP-1 from chimpanzee, orangutan, macaque, and baboon. Cross-reactivity with MCP-1 from other sources has not been determined.
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 11025 Da in size corresponding to the mature human MCP-1 protein by western blotting in appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About CCL2 / MCP1

P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody

CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.

Western blot

Western blot
Anti-Human MCP-1 Antibody - Western Blot. Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E. coli. Recombinant (0.1 ug, 8.6 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
 

Requested From: United States
Date Requested: 12/9/2016

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