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Catalog Number Size Price
LS-C104751-50 50 µg $325 
LS-C104751-100 100 µg $361 
CCL2 / MCP1 Antibody - Western Blot (non-reducing) of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCL2 / MCP1 Antibody - Western Blot (reducing) of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCL2 / MCP1 Antibody - Sandwich ELISA of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCL2 / MCP1 Antibody - Western Blot (non-reducing) of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCL2 / MCP1 Antibody - Western Blot (reducing) of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CCL2 / MCP1 Antibody - Sandwich ELISA of CCL2 / MCP-1 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Rabbit anti‑Rat CCL2 / MCP1 Antibody (WB) LS‑C104751

Polyclonal Rabbit anti‑Rat CCL2 / MCP1 Antibody (WB) LS‑C104751

Note: This antibody replaces LS-C54182, LS-C8624, LS-C16449, LS-C42298
Antibody:
CCL2 / MCP1 Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$325
LS-C104751-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CCL2 / MCP1 Rabbit anti-Rat Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
MCP1 antibody LS-C104751 is an unconjugated rabbit polyclonal antibody to rat MCP1 (CCL2). Validated for ELISA and WB. Cited in 1 publication.
Target
Rat CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
E.coli derived recombinant Rat MCP-1.
Specificity
Rat MCP-1
Applications
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
Usage
Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4% PFA. The primary antibody was incubated at 1 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/ml), a concentration of 4-6 ug/ml of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
Centrifuge the vial before opening. Reconstitute in water to 0.1-1.0 mg/ml. Do not vortex.
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL2 / MCP1
P13500 NM_002982 NP_002973.1

LSBio Ratings

CCL2 / MCP1 Antibody for WB/Western, ELISA LS-C104751 has an LSBio Rating of
Publications (4)

Publications (1)

Renal denervation and CD161a immune ablation prevent cholinergic hypertension and renal sodium retention. Nandita Raikwar, Cameron Braverman, Peter M Snyder, Robert A Fenton, David K Meyerholz, Francois M Abboud, Sailesh C Harwani. American journal of physiology. Heart and circulatory physiology. 2019 September;317:H517-H530.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/25/2022