Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 2D8] (IgG1) to Human CCL2 / MCP1
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Mouse Monoclonal (IgG2a) to Human CCL2 / MCP1
Human CCL2 / MCP1
Human (tested or 100% immunogen sequence identity)
Protein A purified
Specificity and Use
CCL2 / MCP1 antibody was raised against recombinant human MCAF.
Recognizes human MCAF.
Direct ELISA. Note: MCAF does not bind to plastic as good as most proteins-use higher concentration for proper binding. Direct ELISA: 1:5000 dilution will give 0.3 O. D. within 10 minutes using alkaline phosphatase goat anti-mouse Ig.
500 µl deionized water
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.