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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CCL2 / MCP1 Antibody (clone 1F10) LS‑C198160
MCP1 antibody LS-C198160 is an unconjugated mouse monoclonal antibody to human MCP1 (CCL2). Validated for ELISA and WB.
Catalog
Size
Price
LS-C198160-100
100 µg
Unavailable

Popular CCL2 / MCP1 Products

Antibody
Species: Rat
Applications: Western blot, ELISA, Neutralization
Western Blot (non-reducing) of CCL2 / MCP-1 antibody LS-C104751.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Rat
Applications: Western blot, ELISA, Neutralization
Antibody
Species: Mouse
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
Anti-Human MCP-1 Antibody - Western Blot. Anti-human MCP-1 by western blot shows detection of recombinant human MCP-1 raised in E. coli. Recombinant (0.1 ug, 8.6 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Human MCP-1. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (p/n MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
Species: Human
Applications: Western blot, ELISA
IHC of paraffin-embedded liver cancer tissues using CCL2 mouse monoclonal antibody with DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

MCP1 antibody LS-C198160 is an unconjugated mouse monoclonal antibody to human MCP1 (CCL2). Validated for ELISA and WB.
About CCL2 / MCP1
CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody


Specifications

Target
Human CCL2 / MCP1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1F10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
CCL2 / MCP1 antibody was raised against cCL2 (AAH09716, aa1-99 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CCL2 / MCP-1
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.

Western blot

Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CCL2 expression in transfected 293T cell line by CCL2 monoclonal antibody (M05), clone 1F10.Lane 1: CCL2 transfected lysate (Predicted MW: 11 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged CCL2 is 0.1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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