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Anti-CCL2 / MCP1 Antibody (Biotin) LS-C104762

Note: This antibody replaces LS-C54183, LS-C8625, LS-C16448, LS-C42299

Ordering

Wt. Vol. Conc. Price
25 µg - - $285
50 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CCL2 / MCP1 Antibodies

Anti-CCL2 / MCP1 Antibody (aa102-130, clone ECE.2) LS-C139852
Rat Monoclonal [clone ECE.2] (IgG1) to Mouse CCL2 / MCP1
Mouse
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Anti-CCL2 / MCP1 Antibody (clone 2D8) LS-C169178
Mouse Monoclonal [clone 2D8] (IgG1) to Human CCL2 / MCP1
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat CCL2 / MCP1
Rat
IHC - 4% paraformaldehyde, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Biotin Conjugated

Details

Rat CCL2 / MCP1
Rabbit
Rat (tested or 100% immunogen sequence identity)
Polyclonal
Biotin. Also available Unconjugated.
Immunoaffinity purified
Unmodified

Applications

  • IHC - 4% paraformaldehyde
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (1 µg/ml)
  • Neutralization
  • (applications tested for the base form of this product only)

Specificity and Use

CCL2 / MCP1 antibody was raised against e.coli derived recombinant Rat MCP-1.
Rat MCP-1
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat MCP-1(MCAF) by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat MCP-1(MCAF) (LS-C104751) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile PBS containing 0.1% BSA
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About CCL2 / MCP1

P13500 NM_002982 NP_002973.1

CCL2 Antibody, C-C motif chemokine 2 Antibody, Chemokine (C-C motif) ligand 2 Antibody, GDCF-2 Antibody, HC11 Antibody, HSMCR30 Antibody, MCP1 Antibody, SCYA2 Antibody, Small-inducible cytokine A2 Antibody, Monocyte secretory protein JE Antibody, MCAF Antibody, MCP-1 Antibody, Monocyte chemotactic protein 1 Antibody, SMC-CF Antibody

CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues.

Immunohistochemistry

Immunohistochemistry
IHC of CCL2 / MCP-1 antibody LS-C104751. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of CCL2 / MCP-1 antibody LS-C104751. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of CCL2 / MCP-1 antibody LS-C104751. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of CCL2 / MCP-1 antibody LS-C104762 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Direct ELISA of CCL2 / MCP-1 antibody LS-C104762 This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of CCL2 / MCP-1 antibody LS-C104751. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/8/2016

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