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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP8 / Caspase 8 Antibody (N‑Terminus) LS‑C31148
Caspase 8 antibody LS-C31148 is an unconjugated rabbit polyclonal antibody to Caspase 8 (CASP8) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C31148-100
100 µg (1 mg/ml)
Unavailable

Popular CASP8 / Caspase 8 Products

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CASP8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Anti-Caspase 8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3796 concentration 5 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: Immunoprecipitation
Western blot Image
Species: Human
Applications: Western blot
Western blot of extracts from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right was incubated with synthetic peptide.
Species: Human
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

Caspase 8 antibody LS-C31148 is an unconjugated rabbit polyclonal antibody to Caspase 8 (CASP8) from human, mouse, rat and other species. Validated for WB.
About CASP8 / Caspase 8
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Q14790 nm_001228 AAC50645.1

CASP8 Antibody, ALPS2B Antibody, Apoptotic protease Mch-5 Antibody, Apoptotic cysteine protease Antibody, Casp-8 Antibody, Caspase-8 Antibody, CAP4 Antibody, Caspase 8 Antibody, MACH Antibody, MCH5 Antibody, MORT1-associated ced-3 homolog Antibody, ICE-like apoptotic protease 5 Antibody, Pro Caspase 8 Antibody, FADD-like ICE Antibody, FLICE Antibody, MACH-alpha-1/2/3 protein Antibody, MACH-beta-1/2/3/4 protein Antibody


Specifications

Target
Human CASP8 / Caspase 8
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Horse (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide from N-Terminus of human CFLAR (O15519, NP_003870). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Marmoset, Rat, Bovine (100%); Galago, Elephant, Horse (93%); Dog, Bat (86%); Mouse (80%).
Blocking Peptide
LS-E10610 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C31148. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human CASP8 / Caspase 8
Presentation
Lyophilized.
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HepG2 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HepG2 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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