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Catalog Number Size Price
LS-C746976-20 20 µl $275 
LS-C746976-100 100 µl (0.456 mg/ml) $389 
CASP8 / Caspase 8 Antibody - Immunofluorescence analysis of U2OS cells using CASP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CASP8 / Caspase 8 Antibody - Western blot analysis of extracts of various cell lines, using CASP8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
CASP8 / Caspase 8 Antibody - Immunofluorescence analysis of U2OS cells using CASP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
CASP8 / Caspase 8 Antibody - Western blot analysis of extracts of various cell lines, using CASP8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
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Polyclonal Rabbit anti‑Human CASP8 / Caspase 8 Antibody (IF, WB) LS‑C746976

Polyclonal Rabbit anti‑Human CASP8 / Caspase 8 Antibody (IF, WB) LS‑C746976

Antibody:
CASP8 / Caspase 8 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C746976-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
CASP8 / Caspase 8 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Caspase 8 antibody LS-C746976 is an unconjugated rabbit polyclonal antibody to human Caspase 8 (CASP8). Validated for IF and WB.
Target
Human CASP8 / Caspase 8
Synonyms
CASP8 | ALPS2B | Apoptotic protease Mch-5 | Apoptotic cysteine protease | Casp-8 | Caspase-8 | CAP4 | Caspase 8 | MACH | MCH5 | MORT1-associated ced-3 homolog | ICE-like apoptotic protease 5 | Pro Caspase 8 | FADD-like ICE | FLICE | MACH-alpha-1/2/3 protein | MACH-beta-1/2/3/4 protein
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-479 of human CASP8 (NP_001073593.1). MDFSRNLYDIGEQLDSEDLASLKFLSLDYIPQRKQEPIKDALMLFQRLQEKRMLEESNLSFLKELLFRINRLDLLITYLNTRKEEMERELQTPGRAQISAYRVMLYQISEEVSRSELRSFKFLLQEEISKCKLDDDMNLLDIFIEMEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQDSESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLDAGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIPVETDSEEQPYLEMDLSSPQTRYIPDEAD
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
Usage
The predicted MW is 25-32kDa/45- 61kDa, while the observed MW by Western blot was 55kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CASP8 / Caspase 8
Q14790 nm_001228 AAC50645.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/9/2024