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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CASP8 / Caspase 8

caspase 8, apoptosis-related cysteine peptidase

Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Gene Name: caspase 8, apoptosis-related cysteine peptidase
Family/Subfamily: Protease , Cysteine C14
Synonyms: CASP8, ALPS2B, Apoptotic protease Mch-5, Apoptotic cysteine protease, Casp-8, Caspase-8, CAP4, Caspase 8, MACH, MCH5, MORT1-associated ced-3 homolog, ICE-like apoptotic protease 5, Pro Caspase 8, FADD-like ICE, FLICE, MACH-alpha-1/2/3 protein, MACH-beta-1/2/3/4 protein
Target Sequences: nm_001228 AAC50645.1 Q14790

Filtered by: None
Target
CASP8 / Caspase 8 (176)
Reactivity
Human (138)
Mouse (61)
Rat (45)
Bovine (22)
Chimpanzee (3)
Cow (16)
Dog (5)
Gerbil (3)
Gibbon (3)
Monkey (11)
Non-Human Primate (1)
Orangutan (3)
Application
IHC (88)
IHC-Fr (2)
IHC-P (22)
WB (164)
Flo (8)
DB (1)
ELISA (25)
ICC (14)
IF (8)
IP (32)
Peptide-ELISA (4)
Host
rabbit (133)
mouse (39)
rat (4)
Isotype
IgG (46)
IgG1 (18)
IgG1,k (4)
IgG2a (1)
IgG2b (4)
IgG2b,k (1)
IgG3,k (1)
IgM (1)
Clonality
monoclonal mc (44)
polyclonal pc (132)
Clone
1G12 (1)
1H11 (2)
2G12 (1)
3B10 (1)
550CT8.5.2 (1)
5D3 (1)
5F7 (1)
8CSP03 (2)
90A992 (2)
C15 (1)
FLICE 4-1-20 (1)
G1/G1-4 (1)
SB125a (1)
Format
AP Conjugated (2)
APC (8)
APC Conjugated (10)
Azide-free (1)
Biotin Conjugated (17)
Cy3 Conjugated (8)
Cy7 Conjugated (8)
FITC Conjugated (15)
HRP Conjugated (13)
Magnetic beads Conjugated (1)
PE Conjugated (10)
Sepharose Conjugated (1)
Unconjugated (91)
Epitope
aa25-175 (16)
aa7-216 (10)
aa8-197 (10)
aa1-218 (8)
aa217-384 (8)
aa219-376 (8)
aa385-479 (8)
C-Terminus (7)
Internal (6)
N-Terminus (6)
pSer347 (3)
pTyr380 (3)
290-370 aa (2)
Thr341 (2)
aa181-478 (2)
aa217-222 (2)
aa432-461 (2)
310-390 aa, C-terminal (1)
320-400 aa (1)
aa240-259 (1)
aa257-277 (1)
aa313-362 (1)
aa335-384 (1)
aa388-480 (1)
aa458-473 (1)
pThr341 (1)
Publications
No (175)
Yes (1)
Showing 1-25 of 176 results

CASP8 / Caspase 8 Antibody IHC-plus™ LS-B3796
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Mouse, Rat, Human
ELISA ICC IF IHC IHC-P WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-Caspase 8 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 8 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

CASP8 / Caspase 8 Antibody (aa432-461) LS-C99287
Publications (1)
Publications (1)
Rabbit Polyclonal to Human CASP8 / Caspase 8
Mouse, Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CASP8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence of CASP8 Antibody with HeLa cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

CASP8 / Caspase 8 Antibody (clone 5F7) LS-C179764
Mouse Monoclonal [clone 5F7] (IgG2b) to Human CASP8 / Caspase 8
Human
WB
Unconjugated
100µg 1mg/ml  $355.00
Western blot Image

CASP8 / Caspase 8 Antibody (Internal, Sepharose) LS-C171973
Rabbit Polyclonal to Human CASP8 / Caspase 8
Mouse, Rat, Human
IP WB
Sepharose Conjugated
100µg  $325.00
 This image was taken for the unconjugated form of this product. Other forms have not been tested.

CASP8 / Caspase 8 Antibody (phospho-Tyr380) LS-C351928
Rabbit Polyclonal to Human CASP8 / Caspase 8
Rat, Human, Monkey
WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot analysis of Caspase 8 (pY380) expression in HEK293T PMA-treated (A); Jurkat etoposide-treated (B); HeLa PMA-treated (C); H9C2 PMA-treated (D) whole cell lysates.

CASP8 / Caspase 8 Antibody (aa7-216) LS-C374044
Rabbit Polyclonal to Human CASP8 / Caspase 8
Human
ICC IHC IP WB
Unconjugated
20µl 0.5mg/ml  $195.00; 100µl 0.5mg/ml  $235.00; 200µl 0.5mg/ml  $275.00; 1ml 0.5mg/ml  $445.00; 10ml 0.5mg/ml  $1,680.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

CASP8 / Caspase 8 Antibody (Internal) IHC-plus™ LS-B12449
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Human
ICC IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

CASP8 / Caspase 8 Antibody (clone FLICE 4-1-20) IHC-plus™ LS-B240
Mouse Monoclonal [clone FLICE 4-1-20] (IgG1,k) to Human CASP8 / Caspase 8
Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-Caspase 8 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.

CASP8 / Caspase 8 Antibody (aa181-478, clone C15) LS-C344789
Mouse Monoclonal [clone C15] (IgG2b) to Human CASP8 / Caspase 8
Human
ICC IP WB
Unconjugated
50µg  $305.00; 100µg  $395.00

CASP8 / Caspase 8 Antibody LS-C746976
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Human
IF WB
Unconjugated
50µl  $235.00
Immunofluorescence analysis of U2OS cells using CASP8 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using CASP8 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

CASP8 / Caspase 8 Antibody (clone 3B10) LS-C344799
Rat Monoclonal [clone 3B10] (IgG1,k) to Mouse CASP8 / Caspase 8
Mouse
ELISA Flo ICC IHC WB
Unconjugated
100µg  $365.00
Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
Western blot using anti-Caspase-8 (mouse), mAb (3B10) detecting the cleaved active p20 subunit of mouse caspase-8 in addition to the caspase-8 precursor, upon an apoptotic stimulus e.g. by cross-linked rhsFasL.

CASP8 / Caspase 8 Antibody LS-C661858
Mouse Monoclonal to Human CASP8 / Caspase 8
Human
Flo ICC IHC IHC-P WB
Unconjugated
100µg  $355.00
Immunohistochemistry - Anti-Caspase 8 Picoband Antibody
Immunohistochemistry - Anti-Caspase 8 Picoband Antibody

CASP8 / Caspase 8 Antibody (phospho-Tyr380) LS-C198731
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from LOVO cells treated with UV 15', using Caspase 8 (Phospho-Tyr380) Antibody. The lane on the right is blocked with the phospho peptide.

CASP8 / Caspase 8 Antibody (clone 1G12) LS-C344953
Rat Monoclonal [clone 1G12] (IgG1,k) to Mouse CASP8 / Caspase 8
Mouse
ELISA Flo ICC WB
Unconjugated
100µg  $365.00
Western blot using anti-Caspase-8 (mouse), mAb (1G12) detecting endogenous caspase-8 in various mouse cell line, but not in human cell lines.
Western blot using anti-Caspase-8 (mouse), mAb (1G12) detecting endogenous caspase-8 in MEFs from WT mice, but not in MEFs from caspase-8-/- mice. Several smaller bands detected in the caspase-8-/- MEFs, correspond to truncated forms of caspase-8 made in the caspase-8-/- mice since only exons 1 and 2 of mouse caspase-8 were deleted in these knock-out mice and not the region encoding the p20 subunit. Note: Extra bands marked by * are only seen in lysates from caspase-8-/- MEFs and not in lysates from any WT cell lines or mouse WT tissue.

CASP8 / Caspase 8 Antibody (clone 1H11) LS-C169428
Mouse Monoclonal [clone 1H11] (IgG1) to Human CASP8 / Caspase 8
Human
ELISA Flo IHC WB
Unconjugated
100µl  $365.00
IHC of paraffin-embedded colon cancer tissues using CASP8 mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded liver cancer tissues using CASP8 mouse monoclonal antibody with DAB staining.

CASP8 / Caspase 8 Antibody (aa313-362) IHC-plus™ LS-B12516
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

CASP8 / Caspase 8 Antibody (aa217-384) LS-C293219
Rabbit Polyclonal to Human CASP8 / Caspase 8
Human
ICC IHC IP WB
Unconjugated
20µl 0.5mg/ml  $195.00; 100µl 0.5mg/ml  $235.00; 200µl 0.5mg/ml  $275.00; 1ml 0.5mg/ml  $445.00; 10ml 0.5mg/ml  $1,680.00
Immunohistochemistry - Paraffin Image
Immunohistochemistry - Paraffin Image

CASP8 / Caspase 8 Antibody LS-C659697
Mouse Monoclonal (IgG1) to Human CASP8 / Caspase 8
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $405.00

CASP8 / Caspase 8 Antibody LS-C748041
Rabbit Monoclonal (IgG) to Human CASP8 / Caspase 8
Human
WB
Unconjugated
50µl  $265.00

CASP8 / Caspase 8 Antibody IHC-plus™ LS-B13015
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Human
ELISA IHC IHC-P WB
Unconjugated
50µg  $425.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CASP8 / Caspase 8 Antibody LS-C659694
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $405.00

CASP8 / Caspase 8 Antibody LS-C393189
Rabbit Polyclonal to Mouse CASP8 / Caspase 8
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
100µl  $325.00

CASP8 / Caspase 8 Antibody (clone 1H11) LS-C758974
Mouse Monoclonal [clone 1H11] (IgG1) to Human CASP8 / Caspase 8
Mouse, Rat, Non-Human Primate, Human
Flo IHC WB
Unconjugated
100µl  $445.00
Caspase 8 Antibody in Immunohistochemistry (IHC)
Caspase 8 Antibody in Immunohistochemistry (IHC)

CASP8 / Caspase 8 Antibody IHC-plus™ LS-B3797
Rabbit Polyclonal (IgG) to Human CASP8 / Caspase 8
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
0.05mg 1mg/ml  $425.00
Anti-Caspase 8 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Caspase 8 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

CASP8 / Caspase 8 Antibody LS-C148246
Rabbit Polyclonal to Human CASP8 / Caspase 8
Human
IHC IHC-Fr IHC-P IP WB
Unconjugated
50µl  $335.00
Formalin-fixed, paraffin-embedded sections from a brain tumor tissue array stained for Caspase-8 expression using Polyclonal Antibody to Caspase-8 (Pro and Active) at 1:2000. A. Anaplastic glioma cores from two different patients, positive (left) and negative (right) staining for caspase-8. B. Higher magnification from the caspase-8 positive (A, left) core.
Showing 1-25 of 176 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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