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Anti-c-Kit / CD117 Antibody (aa26-520) LS-C150051

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular c-Kit / CD117 Antibodies

Anti-c-Kit / CD117 Antibody (Extracellular Domain) IHC-plus™ LS-A9389
Rabbit Polyclonal to Human c-Kit / CD117
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-c-Kit / CD117 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A9391
Rabbit Polyclonal to Human c-Kit / CD117
Human, Monkey, Bat, Bovine, Dog, Goat, Horse, Pig, Rabbit
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-c-Kit / CD117 Antibody LS-C78828
Rabbit Polyclonal (IgG) to Human c-Kit / CD117
Human, Rat
IHC, Immunofluorescence, Flow Cytometry
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human c-Kit / CD117
Human
IHC - Paraffin, Western blot, Flow Cytometry, Neutralization
Unconjugated

Details

Human c-Kit / CD117
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry
  • Neutralization

Specificity and Use

c-Kit / CD117 antibody was raised against s. frugiperda insect ovarian cell line Sf21-derived recombinant human SCF R/ckit Gln26-Thr520 Accession # P10721.
aa26-520
Detects human and mouse SCF R/ckit in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with.
Western Blot, 0.1 µg/ml, Recombinant Human SCF R/c-kit and Recombinant Mouse SCF R/c-kit. Flow Cytometry, 2.5 µg/106 cells, TF-1 human erythroleukemic cell line. Immunohistochemistry, 5-15 µg/ml, Immersion fixed paraffin-embedded sections of human brain (hippocampus) subjected to. Antigen Retrieval Reagent-Basic. Neutralization, Measured by its ability to neutralize SCF/c-kit Ligand-induced proliferation in the TF-1 human erythroleukemic cell. line. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/ml in the presence of 4 ng/ml Recombinant Human SCF/c-kit Ligand.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
500 µl Reconstitute with sterile PBS.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About c-Kit / CD117

P10721 NM_000222 NP_000213.1

KIT Antibody, C-Kit Antibody, CD117 Antibody, PBT Antibody, Piebald trait Antibody, Piebald trait protein Antibody, Proto-oncogene c-Kit Antibody, SCFR Antibody, Soluble KIT variant 1 Antibody, Tyrosine-protein kinase Kit Antibody, p145 c-kit Antibody, CD117 antigen Antibody

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL.

Neutralization

Neutralization
Cell Proliferation Induced by SCF/c-kit Ligand and Neutralization by Human SCF R/c-kit Antibody. Recombinant Human SCF/ckit Ligand stimulates proliferation in the TF1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/ckit Ligand (4 ng/ml) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF R/ckit Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.3-1.0 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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