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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B6058-50 50 µg $495 
c-Kit / CD117 Antibody - Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
c-Kit / CD117 Antibody - Western blot of KIT expression in transfected 293T cell line by KIT monoclonal antibody, clone 5F6.
c-Kit / CD117 Antibody - Detection limit for recombinant GST tagged KIT is 0.03 ng/ml as a capture antibody.
c-Kit / CD117 Antibody - Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
c-Kit / CD117 Antibody - Western blot of KIT expression in transfected 293T cell line by KIT monoclonal antibody, clone 5F6.
c-Kit / CD117 Antibody - Detection limit for recombinant GST tagged KIT is 0.03 ng/ml as a capture antibody.
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IHC‑plus™ Monoclonal Mouse anti‑Human c‑Kit / CD117 Antibody (clone 5F6, IHC, WB) LS‑B6058

IHC‑plus™ Monoclonal Mouse anti‑Human c‑Kit / CD117 Antibody (clone 5F6, IHC, WB) LS‑B6058

Note: This antibody replaces LS-C133899
Antibody:
c-Kit / CD117 Mouse anti-Human Monoclonal (5F6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$495
LS-B6058-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
c-Kit / CD117 Mouse anti-Human Monoclonal (5F6) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD117 antibody LS-B6058 is an unconjugated mouse monoclonal antibody to human CD117 (c-Kit). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human c-Kit / CD117
Synonyms
KIT | C-Kit | CD117 | PBT | Piebald trait | Piebald trait protein | Proto-oncogene c-Kit | SCFR | Soluble KIT variant 1 | Tyrosine-protein kinase Kit | p145 c-kit | CD117 antigen
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
5F6
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
KIT (NP_000213, 41 aa ~ 140 aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human c-Kit
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates and the recombinant protein used as the immunogen.
Presentation
PBS, pH 7.4
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-Kit / CD117
P10721 NM_000222 NP_000213.1

Validation

c-Kit / CD117 Antibody - Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
Anti-c-Kit antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.

LSBio Ratings

IHC-plus™ c-Kit / CD117 Antibody (clone 5F6) for IHC, WB/Western, ELISA LS-B6058 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/4/2020