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Catalog Number Size Price
LS-C357447-10 10 µg $318 
LS-C357447-100 100 µg (0.5 mg/ml) $470 
c-Kit / CD117 Antibody - C-Kit antibody IHC-paraffin: Human Intestinal Cancer Tissue.
c-Kit / CD117 Antibody - C-Kit antibody Western blot. All lanes: Anti C-Kit at 0.5 ug/ml. WB: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 109 kD. Observed band size: 109 kD.
c-Kit / CD117 Antibody - C-Kit antibody Western blot. All lanes: Anti C-Kit at 0.5 ug/ml. WB: Recombinant Human C-Kit Protein 0.5ng. Predicted band size: 49 kD. Observed band size: 49 kD.
c-Kit / CD117 Antibody - Flow Cytometry analysis of K562 cells using anti-C-Kit antibody. Overlay histogram showing K562 cells stained with anti-C-Kit antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
c-Kit / CD117 Antibody - C-Kit antibody IHC-paraffin: Human Intestinal Cancer Tissue.
c-Kit / CD117 Antibody - C-Kit antibody Western blot. All lanes: Anti C-Kit at 0.5 ug/ml. WB: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 109 kD. Observed band size: 109 kD.
c-Kit / CD117 Antibody - C-Kit antibody Western blot. All lanes: Anti C-Kit at 0.5 ug/ml. WB: Recombinant Human C-Kit Protein 0.5ng. Predicted band size: 49 kD. Observed band size: 49 kD.
c-Kit / CD117 Antibody - Flow Cytometry analysis of K562 cells using anti-C-Kit antibody. Overlay histogram showing K562 cells stained with anti-C-Kit antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C-Kit Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human c‑Kit / CD117 Antibody (aa26‑285, IHC, WB) LS‑C357447

Polyclonal Rabbit anti‑Human c‑Kit / CD117 Antibody (aa26‑285, IHC, WB) LS‑C357447

Note: This antibody replaces LS-C388093
Antibody:
c-Kit / CD117 Rabbit anti-Human Polyclonal (aa26-285) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357447-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
c-Kit / CD117 Rabbit anti-Human Polyclonal (aa26-285) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD117 antibody LS-C357447 is an unconjugated rabbit polyclonal antibody to human CD117 (c-Kit) (aa26-285). Validated for IHC and WB.
Target
Human c-Kit / CD117
Synonyms
KIT | C-Kit | CD117 | PBT | Piebald trait | Piebald trait protein | Proto-oncogene c-Kit | SCFR | Soluble KIT variant 1 | Tyrosine-protein kinase Kit | p145 c-kit | CD117 antigen
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.
Epitope
aa26-285
Specificity
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About c-Kit / CD117
P10721 NM_000222 NP_000213.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024