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Anti-BUB1 Antibody (N-Terminus) IHC-plus™ LS-B2108

Note: This antibody replaces LS-C34703

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BUB1 Antibodies

Anti-BUB1 Antibody (N-Terminus) IHC-plus™ LS-B2108
Rabbit Polyclonal (IgG) to Human BUB1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-BUB1 Antibody (aa1-30) LS-C156894
Rabbit Polyclonal to Human BUB1
Human
Western blot, Flow Cytometry
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BUB1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human BUB1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (4 µg/ml)

Specificity and Use

BUB1 antibody was raised against synthetic peptide from human BUB1.
LS-E30351 - Liquid - 50 µg - $145.00
N-Terminus
a 19 amino acid peptide from near the amino terminus of human Bub1
Immunohistochemistry: LS-B2108 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2108 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About BUB1

O43683 NM_004336 NP_004327.1

BUB1 Antibody, Bub1A Antibody, BUB1L Antibody, HBUB1 Antibody

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion.

Immunohistochemistry

Immunohistochemistry
Anti-BUB1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2108 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of Bub1 in A20 cells with Bub1 antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/ml.

Western blot

Western blot
Western blot of bub1 in A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/ml.

Requested From: United States
Date Requested: 12/4/2016

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