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Anti-BRAF / B-Raf Antibody (phospho-Thr598/Ser601) IHC-plus™ LS-B1627

Note: This antibody replaces LS-C41172


Wt. Vol. Conc. Price
- 50 µl 0.5 mg/ml $395

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Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
IHC - Paraffin, Immunofluorescence


Human BRAF / B-Raf
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified


IHC - Paraffin (1:100), Immunofluorescence

Specificity and Use

BRAF / B-Raf antibody was raised against synthetic peptide from human BRAF.
Modified peptide
Immunohistochemistry: LS-B1627 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1627 was determined to be 1:100.


Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody

BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance.


Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1627 dilution 1:100.


Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against Thr598/Ser601 phosphorylated B-Raf, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.

Requested From: United States
Date Requested: 12/1/2015

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