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Anti-BRAF / B-Raf Antibody (aa754-765) LS-C4218

Catalog Size Price
LS-C4218-100 100 µg (1 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Human, Monkey, Dog, Horse
Western blot (applications tested for the base form of this product only)


Human BRAF / B-Raf
Human, Monkey, Dog, Horse (tested or 100% immunogen sequence identity)
Mouse, Rabbit, Chicken (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified


  • Western blot (1 - 2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

BRAF / B-Raf antibody was raised against synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%); Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%); Bat (83%).
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 ug.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody

BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Requested From: 
Date Requested: 3/20/2018

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