B-Raf antibody LS-C4218 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human, dog, horse and other species. Validated for WB.
Human BRAF / B-Raf
Human, Monkey, Dog, Horse
(tested or 100% immunogen sequence identity)
Mouse, Rabbit, Chicken (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
BRAF / B-Raf antibody was raised against synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%); Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%); Bat (83%).
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 ug.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.