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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRAF / B‑Raf Antibody (aa754‑765) LS‑C4218
B-Raf antibody LS-C4218 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human, dog, horse and other species. Validated for WB.
Antibody
Catalog
Size
Price
LS-C4218-100
100 µg (1 mg/ml)
$425

Popular BRAF / B-Raf Products

Anti-BRAF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1627 dilution 1:100.
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence
Anti-BRAF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3862 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Anti-BRAF antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3444 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
All lanes: Anti B Raf at 0.5 ug/ml. Lane 1: Rat Testis Tissue at 50 ug. Lane 2: Rat Brain Tissue at 50 ug. Lane 3: Mouse Testis Tissue at 50 ug. Lane 4: Mouse Brain Tissue at 50 ug. Lane 5: HeLa Whole Cell Lysate at 40 ug. Lane 6: Jurkat Whole Cell Lysate at 40 ug. Lane 7: MCF-7 Whole Cell Lysate at 40 ug. Lane 8: K562 Whole Cell Lysate at 40 ug. Predicted band size: 84 kD . Observed band size: 84 kD.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

B-Raf antibody LS-C4218 is an unconjugated rabbit polyclonal antibody to B-Raf (BRAF) from human, dog, horse and other species. Validated for WB.

Specifications

Target
Human BRAF / B-Raf
Synonyms
BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1
Host
Rabbit
Reactivity
Human, Monkey, Dog, Horse (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
BRAF / B-Raf antibody was raised against synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%); Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%); Bat (83%).
Epitope
aa754-765
Specificity
Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.
Usage
Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 ug/ml detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 ug.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BRAF / B-Raf
BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. P15056 NM_004333 NP_004324.2


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Requested From: United States
Date Requested: 10/22/2018

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