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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BCAR3 Antibody (N‑Terminus) LS‑C153636
BCAR3 antibody LS-C153636 is an unconjugated rabbit polyclonal antibody to human BCAR3. Validated for WB.
Catalog
Size
Price
LS-C153636-100
100 µg
Unavailable

Popular BCAR3 Products

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Immunohistochemical analysis of BCAR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

BCAR3 antibody LS-C153636 is an unconjugated rabbit polyclonal antibody to human BCAR3. Validated for WB.
About BCAR3
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. O75815 NM_003567 NP_003558.1

BCAR3 Antibody, NSP2 Antibody, SH2D3B Antibody, Novel SH2-containing protein 2 Antibody


Specifications

Target
Human BCAR3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
Blocking Peptide
LS-E31318 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-C153636. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
Usage
BCAR3 antibody can be used for detection of BCAR3 by Western blot at 1-2 ug/mL.
Presentation
PBS, 0.02% sodium azide
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 µg/mL.

Immunofluorescence

Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.
Immunofluorescence of BCAR3 in human kidney tissue with BCAR3 antibody at 20 ug/ml.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 µg/mL.

Western blot

Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.
Western blot analysis of BCAR3 in HeLa cell lysate with BCAR3 antibody at (A) 1 and (B) 2 ug/ml.


Requested From: 
Date Requested: 6/25/2018

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