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Anti-BBC3 / PUMA Antibody LS-C779

Ordering

Wt. Vol. Conc. Price
100 µg 200 µl 0.5 mg/ml $335
Inquire for larger quantities

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Rabbit Polyclonal (IgG) to Human BBC3 / PUMA
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human BBC3 / PUMA
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Protein G purified

Applications

IHC - Paraffin, Western blot (1:500 - 1:1000)

Specificity and Use

BBC3 / PUMA antibody was raised against a portion of amino acids 186-199 (PLPRGHRAPEMEPN ) of human PUMA a. Percent identity by BLAST analysis: Human, Monkey (100%); Marmoset (93%); Ferret, Panda, Dog, Horse, Pig (86%); Mouse, Rat (79%).
The amino acid sequence used as immunogen is 100% homologous in human (PUMA a and b) and 78% homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.

Packaging

PBS, 0.02% sodium azide.
Long term: -70°C; Short term: +4°C
For research use only.

About BBC3 / PUMA

Q9BXH1 NM_014417 NP_055232.1

BBC3 Antibody, Bcl-2-binding component 3 Antibody, BCL2 binding component 3 Antibody, JFY-1 Antibody, JFY1 Antibody, PUMA Antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53.

Immunohistochemistry

Immunohistochemistry
PUMA expression in human dysplastic nevi (premalignant melanoma).

Western blot

Western blot
Western blot analysis for Puma a(CT) using antibody at 1:500 against 30 ug/lane of A)Jurkat and B) NIH 3T3whole cell lysate.

Requested From: United States
Date Requested: 9/30/2016

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