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Anti-BAX Antibody (clone ARBX-1, Ready-to-Use) LS-C392006

Catalog Size Price
LS-C392006-7000 7000 µl Unavailable

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100% Guaranteed
Mouse Monoclonal [clone ARBX-1] (IgG1) to Human BAX
Human
IHC - Paraffin, Immunofluorescence, Western blot (applications tested for the base form of this product only)
Unconjugated, Ready-to-Use

Details

Human BAX
Mouse
Human (tested or 100% immunogen sequence identity)
Mouse, Rat
IgG1 Monoclonal [ARBX-1]
Unconjugated
Protein G purified
Ready-to-Use. Also available Unmodified or Azide-free.

Applications

  • IHC - Paraffin (1:1)
  • Immunofluorescence
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

BAX antibody was raised against recombinant Full-length Human Protein.
Human BAX
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. BCL2 associated X protein/Bax accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt] Optimal dilution of the Bax antibody should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Packaging

PBS, 0.01% BSA, 0.05% sodium azide
2°C to 8°C
For research use only.

About BAX

Q07812 NM_004324 NP_004315.1

BAX Antibody, BCL2-associated X protein Antibody, Bcl2-L-4 Antibody, Apoptosis regulator BAX Antibody, Bcl-2-like protein 4 Antibody, BCL2L4 Antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death.

Immunohistochemistry

Immunohistochemistry
IHC testing of FFPE Hodgkin's Lymphoma with Bax antibody (clone ARBX-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC testing of FFPE human melanoma with Bax antibody (clone ARBX-1). This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 10/23/2017

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