BAX antibody LS-C3482 is an unconjugated rabbit polyclonal antibody to BAX from human and monkey. Validated for WB.
(tested or 100% immunogen sequence identity)
BAX antibody was raised against a 19 residue synthetic peptide ELALDPVPQDASTKKLSEC based on the human Bax (residues 44-62), KLH coupled. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Panda, Cat (89%); Sheep, Elephant, Dog, Bovine, Horse, Pig (84%).
Detects an ~22kD protein, corresponding to the apparent molecular mass of Bax on SDS-PAGE immunoblots. Additional bands of 40 and 42kD are present in all of these cell lysates and these bands are not inhibited by the relevant peptide. Species cross-reactivity: Human, rat, mouse, bovine, porcine, rabbit and monkey.
Suitable for use in Western Blot. Western Blot (ECL): 1:250. Positive control: HeLa Cell Lysate.
PBS, 0.09% Sodium Azide, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.