Human, Monkey, Bovine, Dog, Goat, Pig
(tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
Protein G purified
Also available Ready-to-Use
- IHC - Paraffin (0.5 - 1 µg/ml)
- Immunofluorescence (1 - 2 µg/ml)
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry (0.5 - 1 µg/10E6 cells)
- (applications tested for the base form of this product only)
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BAX antibody was raised against synthetic peptide (GSGEQPRGGGPTSS, aa3-16 of UniProt Q07812) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Cat, Bovine, Goat, Pig, Dog (100%); Bat, Elephant, Opossum (93%); Rat, Hamster, Guinea pig (86%); Mouse (79%).
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
0.01% BSA, 0.05% sodium azide
Store at 2°C to 8°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.