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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BAX Antibody (aa3‑16, clone SPM336, Azide‑free) LS‑C392387
BAX antibody LS-C392387 is an unconjugated mouse monoclonal antibody to BAX from human, bovine, dog and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C392387-100
100 µg (1 mg/ml)
Unavailable

Popular BAX Products

Anti-BAX antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1949 dilution 1:50.
Species: Mouse, Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Pig, Rabbit
Applications: Western blot
Anti-BAX antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2510 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunoprecipitation
Antibody
Species: Rat
Applications: Western blot, Immunoprecipitation
Anti-BAX antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6546 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Product Description

BAX antibody LS-C392387 is an unconjugated mouse monoclonal antibody to BAX from human, bovine, dog and other species. Validated for Flow, IF, IHC and WB.
About BAX
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Q07812 NM_004324 NP_004315.1

BAX Antibody, BCL2-associated X protein Antibody, Bcl2-L-4 Antibody, Apoptosis regulator BAX Antibody, Bcl-2-like protein 4 Antibody, BCL2L4 Antibody


Specifications

Target
Human BAX
Host
Mouse
Reactivity
Human, Monkey, Bovine, Dog, Goat, Pig (tested or 100% immunogen sequence identity)
Predicted
Bat (at least 90% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1 Monoclonal [SPM336]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified or Ready-to-Use.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BAX antibody was raised against synthetic peptide (GSGEQPRGGGPTSS, aa3-16 of UniProt Q07812) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Cat, Bovine, Goat, Pig, Dog (100%); Bat, Elephant, Opossum (93%); Rat, Hamster, Guinea pig (86%); Mouse (79%).
Epitope
aa3-16
Specificity
Human BAX
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
Sterile PBS.
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336). This image was taken for the unmodified form of this product. Other forms have not been tested.

Western blot

Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.
Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa. This image was taken for the unmodified form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/15/2018

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