Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Human BAK1 / BAK
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:1000 - 1:5000)
IHC - Frozen
Western blot (1:1000 - 1:2000)
Immunoprecipitation (1:50 - 1:200)
Specificity and Use
BAK1 / BAK antibody was raised against a synthetic peptide corresponding to amino acids 16-36 (EPALPSASEEQVAQDTEEVFR) of human Bak, GenBank no. NP_001179.1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Orangutan, Monkey (95%); Marmoset, Rat, Ferret, Horse (81%); Mouse (71%).
Antiserum, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
BAK1 Antibody, Apoptosis regulator BAK Antibody, BAK Antibody, BCL2L7 Antibody, Bcl-2-like protein 7 Antibody, BCL2-antagonist/killer 1 Antibody, Bcl2-L-7 Antibody, BCL2-like 7 protein Antibody, CDN1 Antibody, Pro-apoptotic protein BAK Antibody, BAK-LIKE Antibody
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bak, a pro-apoptotic Bcl-2 family member, is an oligomeric protein that localizes to the mitochondria.
Formalin-fixed paraffin-embedded human tissue for Bak expression using LS-C148221 at 1:2000. A. Ductal breast carcinoma. B. Lymphocyte infiltration in soft tissue adjacent to colon carcinoma. Hematoxylin-Eos in counterstain.
Formalin-fixed paraffin-embedded tissue sections of human heart with cardiomyopathy stained for Bak expression using LS-C148221 at 1:2000 (A and B). Hematoxylin-Eosin counterstain.