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Catalog Number Size Price
LS-C800243-100 100 µl (1 mg/ml) $379 
LS-C800243-200 200 µl (1 mg/ml) $421 
BAK1 / BAK Antibody - 1:200 staining human skeletal muscle tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
BAK1 / BAK Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BAK1 / BAK Antibody - Western blot analysis on 293 cell lysates using Bak antibody. The lane on the left is treated with the antigen-specific peptide.
BAK1 / BAK Antibody - 1:200 staining human skeletal muscle tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
BAK1 / BAK Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
BAK1 / BAK Antibody - Western blot analysis on 293 cell lysates using Bak antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human BAK1 / BAK Antibody (IHC, IF, WB) LS‑C800243

Polyclonal Rabbit anti‑Human BAK1 / BAK Antibody (IHC, IF, WB) LS‑C800243

Antibody:
BAK1 / BAK Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800243-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BAK1 / BAK Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
BAK antibody LS-C800243 is an unconjugated rabbit polyclonal antibody to BAK (BAK1) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human BAK1 / BAK
Synonyms
BAK1 | Apoptosis regulator BAK | BAK | BCL2L7 | Bcl-2-like protein 7 | BCL2-antagonist/killer 1 | Bcl2-L-7 | BCL2-like 7 protein | CDN1 | Pro-apoptotic protein BAK | BAK-LIKE
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthesized peptide derived from human Bak
Specificity
Bak antibody detects endogenous levels of total Bak.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAK1 / BAK
Q16611 NM_001188 NP_001179.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024