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Antibodies > BAK1 / BAK > LS-C407698
 
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Catalog Number Size Price
LS-C407698-10 10 µg $318 
LS-C407698-100 100 µg $470 
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Rat Skeletal Muscle Tissue.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
BAK1 / BAK Antibody - Western blot analysis of BAK using anti-BAK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAK at approximately 25KD. The expected band size for BAK is at 23KD.
BAK1 / BAK Antibody - Western blot analysis of BAK using anti-BAK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAK at approximately 25KD. The expected band size for BAK is at 23KD.
BAK1 / BAK Antibody - BAK antibody Western blot. All lanes: Anti BAK at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
BAK1 / BAK Antibody - Flow Cytometry analysis of THP-1 cells using anti-BAK antibody. Overlay histogram showing THP-1 cells stained with anti-BAK antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAK Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in frozen section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Rat Skeletal Muscle Tissue.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Mouse Skeletal Muscle Tissue.
BAK1 / BAK Antibody - IHC analysis of BAK using anti-BAK antibody. BAK was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-BAK Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
BAK1 / BAK Antibody - BAK antibody IHC-paraffin. IHC(P): Human Intestinal Cancer Tissue.
BAK1 / BAK Antibody - Western blot analysis of BAK using anti-BAK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse skeletal muscle tissue lysates, Lane 5: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAK at approximately 25KD. The expected band size for BAK is at 23KD.
BAK1 / BAK Antibody - Western blot analysis of BAK using anti-BAK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human Hela whole cell lysates, Lane 8: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAK at approximately 25KD. The expected band size for BAK is at 23KD.
BAK1 / BAK Antibody - BAK antibody Western blot. All lanes: Anti BAK at 0.5 ug/ml. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 2: Mouse Cardiac Muscle Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 23 kD. Observed band size: 23 kD.
BAK1 / BAK Antibody - Flow Cytometry analysis of THP-1 cells using anti-BAK antibody. Overlay histogram showing THP-1 cells stained with anti-BAK antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAK Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human BAK1 / BAK Antibody (aa22‑211, IHC, WB) LS‑C407698

Polyclonal Rabbit anti‑Human BAK1 / BAK Antibody (aa22‑211, IHC, WB) LS‑C407698

Submit Review LSBio Guarantee
Antibody:
BAK1 / BAK Rabbit anti-Human Polyclonal (aa22-211) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407698-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BAK1 / BAK Rabbit anti-Human Polyclonal (aa22-211) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
BAK antibody LS-C407698 is an unconjugated rabbit polyclonal antibody to BAK (BAK1) (aa22-211) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human BAK1 / BAK
Synonyms
BAK1 | Apoptosis regulator BAK | BAK | BCL2L7 | Bcl-2-like protein 7 | BCL2-antagonist/killer 1 | Bcl2-L-7 | BCL2-like 7 protein | CDN1 | Pro-apoptotic protein BAK | BAK-LIKE
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.
Epitope
aa22-211
Specificity
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAK1 / BAK
Q16611 NM_001188 NP_001179.1

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IHC‑plus™ BAK1 / BAK Antibody (N‑Terminus) LS‑B3333
Species: Human, Mouse, Hamster
Applications: IHC, IHC - Paraffin
BAK1 / BAK Antibody - Anti-BAK1 / BAK antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
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BAK1 / BAK ELISA Kit
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Reactivity: Human
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See all BAK1 / BAK Antibodies

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Requested From: United States
Date Requested: 4/27/2024
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