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Anti-BAD Antibody (phospho-Ser155) LS-C3909

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Wt. Vol. Conc. Price
- 100 µl 0.147 mg/ml $485
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Rabbit Polyclonal (IgG) to Mouse BAD
Mouse, Human
Western blot, ELISA
Unconjugated

Details

Mouse BAD
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein A purified

Applications

Western blot (1:1000), ELISA (1:1000)

Specificity and Use

BAD antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser155 of mouse Bad.
pSer155
Detects overexpressed levels of Bad only when phosphorylated at Ser155. Does not detect Bad phosphorylated at other sites or related family members. Species cross-reactivity: Human.
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. ELISA: 1:1000.

Packaging

10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA 50% glycerol. No preservative added.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About BAD

Q92934 NM_004322 NP_004313.1

BAD Antibody, BBC2 Antibody, Bcl-2-like protein 8 Antibody, BCL2-binding component 6 Antibody, BCL2L8 Antibody, BBC6 Antibody, BCL-X/BCL-2 binding protein Antibody, Bcl-2-binding component 6 Antibody, Bcl2 antagonist of cell death Antibody, BCL2-binding protein Antibody, Bcl2-L-8 Antibody

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.

Requested From: United States
Date Requested: 9/25/2016

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