Target
Mouse BAD
Synonyms
BAD | BBC2 | Bcl-2-like protein 8 | BCL2-binding component 6 | BCL2L8 | BBC6 | BCL-X/BCL-2 binding protein | Bcl-2-binding component 6 | Bcl2 antagonist of cell death | BCL2-binding protein | Bcl2-L-8
Reactivity
Mouse, Human, Monkey, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser112 of mouse Bad.
Specificity
Detects endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species cross-reactivity: Human, mouse, monkey and rat. Other species have not been tested.
Applications
- Western blot (1:2000)
- Immunoprecipitation (1:50)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:2000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee