Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT/HIF-1 beta regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450.
Anti-ARNT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT.
Immunofluorescent staining of HeLa cells using anti-ARNT mouse monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT.
Flow cytometry of Jurkat cells, using anti-ARNT antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometry of HeLa cells, using anti-ARNT antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARNT antibody, and then analyzed by flow cytometry.