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Catalog Number Size Price
LS-C209344-50 50 µg (1.07 mg/ml) $567 
ARHGAP22 / RhoGAP2 Antibody - Western Blot of Rabbit anti-ARHGAP22 pS397 antibody. Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to alanine mutation. Lane 4: NIH3T3 cells transfected with ARHGAP22 S397 to alanine mutaion. Primary antibody: Left: ARHGAP22 pS22, Right: ARHGAP22 pS397 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

Polyclonal Rabbit anti‑Mouse ARHGAP22 / RhoGAP2 Antibody (phospho‑Ser397, IHC, IF, WB) LS‑C209344

Polyclonal Rabbit anti‑Mouse ARHGAP22 / RhoGAP2 Antibody (phospho‑Ser397, IHC, IF, WB) LS‑C209344

Antibody:
ARHGAP22 / RhoGAP2 Rabbit anti-Mouse Polyclonal (pSer397) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209344-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ARHGAP22 / RhoGAP2 Rabbit anti-Mouse Polyclonal (pSer397) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RhoGAP2 antibody LS-C209344 is an unconjugated rabbit polyclonal antibody to RhoGAP2 (ARHGAP22) (pSer397) from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IF, IHC and WB.
Target
Mouse ARHGAP22 / RhoGAP2
Synonyms
ARHGAP22 | RhoGAP2 | RhoGap22
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
Epitope
pSer397
Specificity
This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100% sequence homology. Cross-reactivity with ARHGAP22 pS397 from other sources has not been determined.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1 µg/ml)
  • ELISA (1:20000 - 1:60000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-ARHGAP22 pS397 antibody is useful for ELISA, Western Blot and Immunostaining. Expect a band approximately ~77.8 kD corresponding to the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 1 mg/ml BSA, 50% Glycerol
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ARHGAP22 / RhoGAP2
Q7Z5H3 NM_021226 NP_067049.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024