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Anti-APOA1 / Apolipoprotein A 1 Antibody (aa188-199) IHC-plus™ LS-B2097

Note: This antibody replaces LS-C40556, LS-C11697

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Rabbit Polyclonal (IgG) to Human APOA1 / Apolipoprotein A 1
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human APOA1 / Apolipoprotein A 1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), ICC, Western blot

Specificity and Use

APOA1 / Apolipoprotein A 1 antibody was raised against human ApoA1 protein amino acids 188-199 (RLAEYHAKTEH). Percent identity by BLAST analysis: Gibbon, Marmoset, Panda (82%).
aa188-199
human ApoA1 protein amino acids 188-199 (RLAEYHAKTEH)
Immunohistochemistry: LS-B2097 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2097 was determined to be 5 ug/ml.

Packaging

TBS
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About APOA1 / Apolipoprotein A 1

P02647 NM_000039 NP_000030.1

APOA1 Antibody, ApoA-I Antibody, APOAI Antibody, Apolipoprotein A-I Antibody, Apolipoprotein A1 Antibody, Apo-AI Antibody

APOA1 promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. Synthesized in the liver and small intestine, it consists of two identical chains of 77 amino acids; an 18-amino acid signal peptide is removed co-translationally and a 6-amino acid propeptide is cleaved post-translationally.

Immunohistochemistry

Immunohistochemistry
Anti-Apolipoprotein A I antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2097 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 8/29/2015

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