Mouse APOA1 / Apolipoprotein A 1
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:1000)
- ELISA (1:5000 - 1:10000)
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Apolipoprotein Type A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification
Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross- reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible
Immunohistochemistry: LS-B3641 was validated for use in immunohistochemistry on a panel formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3641 was determined to be 5 ug/ml. Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8.0, 0.01% sodium azide. For extended storage, mix, an equal volume of glycerol.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.
About APOA1 / Apolipoprotein A 1