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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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APG12 / ATG12 Antibody (clone 1F9) LS‑C139421
ATG12 antibody LS-C139421 is an unconjugated mouse monoclonal antibody to human ATG12 (APG12). Validated for ELISA and WB.
Catalog
Size
Price
LS-C139421-100
100 µg
Unavailable

Popular APG12 / ATG12 Products

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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

ATG12 antibody LS-C139421 is an unconjugated mouse monoclonal antibody to human ATG12 (APG12). Validated for ELISA and WB.
About APG12 / ATG12
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. O94817 NM_004707 NP_004698.3

ATG12 Antibody, Autophagy-related protein 12 Antibody, APG12L Antibody, Autophagy related 12 Antibody, FBR93 Antibody, APG12 Antibody, APG12-like Antibody, Ubiquitin-like protein ATG12 Antibody, HAPG12 Antibody


Specifications

Target
Human APG12 / ATG12
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [1F9]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
Immunogen
APG12 / ATG12 antibody was raised against aTG12 (AAH12266, 1 a.a. ~ 141 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human ATG12
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ATG12 is 1 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/19/2018

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