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IHCPlus™ APG12 / ATG12 Antibody Internal Polyclonal ELISA,IHC,IP,WB LS‑B2140

Catalog Number Size Price
LS-B2140-50 50 µg (0.25 mg/ml) $485 
Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHCPlus™ APG12 / ATG12 Antibody Internal Polyclonal ELISA,IHC,IP,WB LS‑B2140

Note: This antibody replaces LS-C11938
Antibody:
Rabbit Polyclonal to Human APG12 / ATG12
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B2140-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human APG12 / ATG12
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
ATG12 antibody LS-B2140 is an unconjugated rabbit polyclonal antibody to ATG12 (APG12 ) from human and mouse. Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human APG12 / ATG12
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide derived from the internal region of the human Apg12 protein.
Epitope
Internal
Specificity
Recognizes human Apg12 protein at ~22kD, as well as additional bands between 40 and 80kD of unknown origin. Reactivity has been confirmed with Apg12-transfected, overexpressed HEK293 cell lysates.
Usage
Immunohistochemistry: LS-B2140 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2140 was determined to be 5 ug/ml. Immunoprecipitation: 7 ug/reaction.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG12 / ATG12
O94817 NM_004707 NP_004698.3

Validation

Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019