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Anti-APG12 / ATG12 Antibody (aa79-128) IHC-plus™ LS-B5897

Note: This antibody replaces LS-C134895


Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Alternate Forms of this Antibody

LS-C465784 Biotin Conjugated
LS-C465785 FITC Conjugated
LS-C465786 HRP Conjugated

Most Popular APG12 / ATG12 Antibodies

Anti-APG12 / ATG12 Antibody (N-Terminus) LS-C53240
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
Western blot
Immunofluorescence Image
Anti-APG12 / ATG12 Antibody (Internal) IHC-plus™ LS-B3216
Rabbit Polyclonal to Human APG12 / ATG12
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-APG12 / ATG12 Antibody LS-C156567
Mouse Monoclonal (IgG2b,k) to Human APG12 / ATG12
IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Rat APG12 / ATG12
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)


Rat APG12 / ATG12
Rat, Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

APG12 / ATG12 antibody was raised against synthetic peptide located between aa79-128 of rat Atg12 (Q2TBJ5, NP_001033584). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%); Zebrafish (85%).
LS-E21596 - Lyophilized - 100 µg - $145.00
Rat ATG12
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.


Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About APG12 / ATG12

O94817 NM_004707 NP_004698.3

ATG12 Antibody, Autophagy-related protein 12 Antibody, APG12L Antibody, Autophagy related 12 Antibody, FBR93 Antibody, APG12 Antibody, APG12-like Antibody, Ubiquitin-like protein ATG12 Antibody, HAPG12 Antibody

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.


Anti-ATG12 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5897 concentration 2.5 ug/ml. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Western blot

Western blot
ATG12 antibody LS-B5897 Western blot of Rat Kidney lysate. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Requested From: United States
Date Requested: 10/22/2016

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