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Anti-APG12 / ATG12 Antibody (aa1-30) LS-C156569

Catalog Size Price
LS-C156569-400 400 µl Unavailable

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Anti-APG12 / ATG12 Antibody (aa1-30) LS-C156569
Rabbit Polyclonal to Human APG12 / ATG12
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IHC - Paraffin, Immunofluorescence, Western blot
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Immunohistochemistry Image

100% Guaranteed Publications (1)
Rabbit Polyclonal to Human APG12 / ATG12
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human APG12 / ATG12
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)

Specificity and Use

LS-E5641 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C156569. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa1-30
This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. Carcinogenesis. 2011 32:964-72. [PubMed:21317300] [PMC:PMC3128555]

About APG12 / ATG12

O94817 NM_004707 NP_004698.3

ATG12 Antibody, Autophagy-related protein 12 Antibody, APG12L Antibody, Autophagy related 12 Antibody, FBR93 Antibody, APG12 Antibody, APG12-like Antibody, Ubiquitin-like protein ATG12 Antibody, HAPG12 Antibody

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescence

Immunofluorescence
Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Western blot

Western blot
Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.

Requested From: 
Date Requested: 12/11/2017

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