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Anti-ALDOA / Aldolase A Antibody LS-C59230

Ordering

Wt. Vol. Conc. Price
- 2000 µl - $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ALDOA / Aldolase A Antibodies

Anti-ALDOA / Aldolase A Antibody IHC-plus™ LS-B1461
Goat Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit, Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ALDOA / Aldolase A Antibody (aa303-331) LS-C98593
Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ALDOA / Aldolase A Antibody (clone 2E6) IHC-plus™ LS-B6377
Mouse Monoclonal [clone 2E6] (IgG2a,k) to Human ALDOA / Aldolase A
Human, Mouse
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit, Human
Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Rabbit ALDOA / Aldolase A
Goat
Rabbit, Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot
  • Immunoprecipitation
  • ELISA (1:3000 - 1:12000)

Specificity and Use

ALDOA / Aldolase A antibody was raised against aldolase [Rabbit Muscle].
Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from
Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. This product has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) catalog no. LS-C60340 and ABTS as a substrate for 30 minutes at room temperature. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at 4°C.
For research use only.

About ALDOA / Aldolase A

P04075 NM_000034 NP_000025.1

ALDOA Antibody, ALDA Antibody, Aldolase Antibody, GSD12 Antibody, Muscle-type aldolase Antibody, Lung cancer antigen NY-LU-1 Antibody

ALDOA / Aldolase A, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle.

Western blot

Western blot
Anti-Aldolase (Rabbit Muscle) Antibody - Western Blot. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% Tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kD consistent with human aldolase.
 

Requested From: United States
Date Requested: 12/4/2016

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