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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ALDOA / Aldolase A

aldolase A, fructose-bisphosphate

ALDOA / Aldolase A, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.

Gene Name: aldolase A, fructose-bisphosphate
Synonyms: ALDOA, ALDA, Aldolase, GSD12, Muscle-type aldolase, Lung cancer antigen NY-LU-1
Target Sequences: NM_000034 NP_000025.1 P04075

Viewing 1-25 of 84 items
ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Goat Polyclonal (IgG) to Rabbit ALDOA / Aldolase A
Rabbit, Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated, Lyophilized
500µg   10mg/ml   $485.00
ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Mouse Monoclonal [clone 2E6] (IgG2a,k) to Human ALDOA / Aldolase A
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   $495.00
ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
200µl   $425.00
ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
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Mouse Monoclonal (IgG1) to Human ALDOA / Aldolase A
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl   1mg/ml   $425.00
ALDOA / Aldolase A Antibody - Immunofluorescent analysis of ALDOA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Rabbit Polyclonal to Human ALDOA / Aldolase A
Rabbit, Mouse, Dog, Rat, Human
ICC, IF, IP, WB
Unconjugated
100µl   1mg/ml   $245.00
Select
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Unconjugated
10mg   $295.00
ALDOA / Aldolase A Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00
Select
Goat Polyclonal (IgG) to Rabbit ALDOA / Aldolase A
Rabbit, Human
ELISA, IHC, WB
HRP Conjugated
100µg   $740.00
Select
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100µg   $405.00
Select
Mouse Monoclonal [clone 1C5B2] (IgG2a) to Human ALDOA / Aldolase A
Human
ELISA, Flo, IHC, WB
Unconjugated
100µl   $365.00
Select
Mouse Monoclonal [clone 2H2B1] (IgG1) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
100µl   $365.00
Select
Mouse Monoclonal [clone PAT3F9AT] (IgG2a,k) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
5µg   $195.00; 20µg   $255.00; 100µg   $475.00
ALDOA / Aldolase A Antibody - The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate
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Mouse Monoclonal [clone AT3F9] (IgG2a,k) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
50µl   1mg/ml   $275.00; 100µl   1mg/ml   $345.00
Select
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Unconjugated
1mg   $485.00
Select
Goat Polyclonal (IgG) to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Biotin Conjugated
100µg   $755.00
Select
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Biotin Conjugated
1ml   $405.00
Select
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
IF, IHC, WB
Unconjugated
100µl   $405.00
ALDOA / Aldolase A Antibody - Western blot analysis of extracts of 293 cells, using ALDOA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
IF, IHC, WB
Unconjugated
100µl   $295.00; 200µl   $385.00
Select
Mouse Monoclonal [clone 3D9-6F3] (IgG2b,k) to Human ALDOA / Aldolase A
Human
ELISA, IF, WB
Unconjugated
100µg   $560.00
Select
Mouse Monoclonal (IgG2b,k) to Human ALDOA / Aldolase A
Human
ELISA, IF, WB
APC Conjugated
100µl   $710.00
ALDOA / Aldolase A Antibody - Immunohistochemistry of paraffin-embedded Human ovarian cancer using ALDOA Polyclonal Antibody at dilution of 1:30.
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Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl   0.3mg/ml   $205.00; 60µl   0.3mg/ml   $235.00; 120µl   0.3mg/ml   $295.00; 200µl   0.3mg/ml   $395.00
ALDOA / Aldolase A Antibody - IHC image of ALDOA Antibody diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Select
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
ELISA, IHC, WB
Unconjugated
50µg   $285.00; 100µg   $385.00
ALDOA / Aldolase A Antibody - Immunofluorescence analysis of A549 cells using ALDOA antibody. Blue: DAPI for nuclear staining.
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Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
ALDOA / Aldolase A Antibody - Western blot of Aldolase A antibody
Select
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
ALDOA / Aldolase A Antibody - Staining COLO205 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Select
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00

Viewing 1-25 of 84 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).