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Immunohistochemistry Services

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ALDOA / Aldolase A

aldolase A, fructose-bisphosphate

ALDOA / Aldolase A, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10.

Gene Name: aldolase A, fructose-bisphosphate
Synonyms: ALDOA, ALDA, Aldolase, GSD12, Muscle-type aldolase, Lung cancer antigen NY-LU-1
Target Sequences: NM_000034 NP_000025.1 P04075

Filtered by: None
Target
ALDOA / Aldolase A (84)
Reactivity
Human (72)
Mouse (33)
Rat (25)
Bat (5)
Bovine (1)
Chimpanzee (3)
Dog (5)
Gibbon (3)
Guinea pig (2)
Hamster (1)
Horse (1)
Monkey (6)
Orangutan (3)
Rabbit (19)
Sheep (1)
Xenopus (2)
Application
IHC (23)
IHC-P (5)
WB (84)
Flo (5)
ELISA (39)
ICC (13)
IF (20)
IP (18)
Peptide-ELISA (4)
Host
rabbit (56)
mouse (14)
goat (11)
sheep (3)
Isotype
IgG (39)
IgG1 (2)
IgG2a (1)
IgG2a,k (3)
IgG2b,k (8)
Clonality
monoclonal mc (14)
polyclonal pc (70)
Clone
1C5B2 (1)
2E6 (1)
2H2B1 (1)
3D9-6F3 (1)
AT3F9 (1)
PAT3F9AT (1)
Format
AP Conjugated (2)
APC (2)
APC Conjugated (4)
Biotin Conjugated (13)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (6)
HRP Conjugated (9)
PE Conjugated (4)
Unconjugated (42)
Epitope
aa18-273 (8)
N-Terminus (6)
1-80 aa, N-terminal (1)
C-Terminus (1)
Thr9 (1)
aa1-365 (1)
aa1-50 (1)
aa303-331 (1)
aa325-375 (1)
aa335-348 (1)
aa50-100 (1)
aa66-95 (1)
aa86-96 (1)
aa9-58 (1)
Publications
No (83)
Yes (1)
Showing 1-25 of 84 results

IHC-plus™ ALDOA / Aldolase A Antibody LS-B1461
Publications (1)
Publications (1)
Goat Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit, Human
ELISA, IF, IHC, IHC-P, IP, WB
Unconjugated
50µg 10mg/ml  $425.00
Anti-Aldolase antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Aldolase antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ ALDOA / Aldolase A Antibody (clone 2E6) LS-B6377
Mouse Monoclonal [clone 2E6] (IgG2a,k) to Human ALDOA / Aldolase A
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $495.00
Anti-Aldolase antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ALDOA monoclonal antibody, clone 2E6. Western blot of ALDOA expression in NIH/3T3.

ALDOA / Aldolase A Antibody (N-Terminus) LS-C351817
Rabbit Polyclonal to Human ALDOA / Aldolase A
Rabbit, Mouse, Dog, Rat, Human
ICC, IF, IP, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunofluorescent analysis of ALDOA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of ALDOA expression in HEK293T (A); HepG2 (B); HeLa (C); NIH3T3 (D); PC12 (E); rat skeletal muscle (F) whole cell lysates.

IHC-plus™ ALDOA / Aldolase A Antibody (aa66-95) LS-B6910
Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
200µl  $425.00
Anti-Aldolase antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Confocal immunofluorescence of Aldolase (ALDOA) Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

ALDOA / Aldolase A Antibody LS-C147305
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Unconjugated
10mg  $285.00

ALDOA / Aldolase A Antibody (aa303-331) LS-C98593
Rabbit Polyclonal to Human ALDOA / Aldolase A
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of anti-ALDOA Antibody in mouse liver tissue lysates (35 ug/lane). ALDOA(arrow) was detected using the purified antibody.

IHC-plus™ ALDOA / Aldolase A Antibody LS-B6425
Mouse Monoclonal (IgG1) to Human ALDOA / Aldolase A
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Aldolase antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 20 ug/ml.
Western blot Image

ALDOA / Aldolase A Antibody (HRP) LS-C34940
Goat Polyclonal (IgG) to Rabbit ALDOA / Aldolase A
Rabbit, Human
ELISA, IHC, WB
HRP Conjugated
100µg  $740.00

ALDOA / Aldolase A Antibody LS-C659331
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
ELISA, Flo, IF, IHC, IP, WB
Unconjugated
100µg  $405.00

ALDOA / Aldolase A Antibody (clone 1C5B2) LS-C682531
Mouse Monoclonal [clone 1C5B2] (IgG2a) to Human ALDOA / Aldolase A
Human
ELISA, Flo, IHC, WB
Unconjugated
100µl  $365.00

ALDOA / Aldolase A Antibody (clone PAT3F9AT) LS-C760654
Mouse Monoclonal [clone PAT3F9AT] (IgG2a,k) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
5µg  $195.00; 20µg  $255.00; 100µg  $475.00

ALDOA / Aldolase A Antibody (clone 2H2B1) LS-C682532
Mouse Monoclonal [clone 2H2B1] (IgG1) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
100µl  $365.00

ALDOA / Aldolase A Antibody (clone AT3F9) LS-C755394
Mouse Monoclonal [clone AT3F9] (IgG2a,k) to Human ALDOA / Aldolase A
Human
ELISA, Flo, ICC, WB
Unconjugated
50µl 1mg/ml  $275.00; 100µl 1mg/ml  $345.00
The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate
ICC/IF analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ALDOA / Aldolase A Antibody LS-C147307
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Unconjugated
1mg  $475.00

ALDOA / Aldolase A Antibody (Biotin) LS-C34939
Goat Polyclonal (IgG) to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Biotin Conjugated
100µg  $755.00

ALDOA / Aldolase A Antibody (Biotin) LS-C147306
Sheep Polyclonal to Rabbit ALDOA / Aldolase A
Rabbit
ELISA, IF, IHC, WB
Biotin Conjugated
1ml  $395.00

ALDOA / Aldolase A Antibody (N-Terminus) LS-C347646
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
IF, IHC, WB
Unconjugated
100µl  $405.00

ALDOA / Aldolase A Antibody LS-C211146
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
ELISA, IHC, WB
Unconjugated
50µg  $285.00; 100µg  $385.00
IHC image of ALDOA Antibody diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot All lanes: Fructose-bisphosphate aldolase A polyclonal antibody at 2µg/ml + EC109 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 40, 46 kDa Observed band size: 40 kDa

ALDOA / Aldolase A Antibody (clone 3D9-6F3) LS-C306205
Mouse Monoclonal [clone 3D9-6F3] (IgG2b,k) to Human ALDOA / Aldolase A
Human
ELISA, IF, WB
Unconjugated
100µg  $560.00

ALDOA / Aldolase A Antibody LS-C496648
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Human
IF, IHC, WB
Unconjugated
100µl  $295.00; 200µl  $385.00
Western blot analysis of extracts of 293 cells, using ALDOA antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

ALDOA / Aldolase A Antibody LS-C403809
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.6mg/ml  $205.00; 60µl 0.6mg/ml  $235.00; 120µl 0.6mg/ml  $295.00; 200µl 0.6mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human gastric cancer using ALDOA Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of paraffin-embedded Human ovarian cancer using ALDOA Polyclonal Antibody at dilution of 1:25.

ALDOA / Aldolase A Antibody LS-C400543
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.2mg/ml  $205.00; 60µl 0.2mg/ml  $235.00; 120µl 0.2mg/ml  $295.00; 200µl 0.2mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human ovarian cancer using ALDOA Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of paraffin-embedded Human gastric cancer using ALDOA Polyclonal Antibody at dilution of 1:30.

ALDOA / Aldolase A Antibody LS-C335133
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunofluorescence analysis of A549 cells using ALDOA antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ALDOA antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 2s.

ALDOA / Aldolase A Antibody (1-80 aa, N-terminal) LS-C381979
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Aldolase A antibody
Western blot of Aldolase A antibody

ALDOA / Aldolase A Antibody LS-C804459
Rabbit Polyclonal (IgG) to Human ALDOA / Aldolase A
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunofluorescence Image
Western blot Image
Showing 1-25 of 84 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).