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Catalog Number	Size	Price
LS-B1461-500	500 µg (10 mg/ml)	$485

ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

ALDOA / Aldolase A Antibody - Anti-Aldolase antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

ALDOA / Aldolase A Antibody - Anti-Aldolase Antibody - Western Blot. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% Tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4° C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kD consistent with human aldolase.

ALDOA / Aldolase A Antibody - Anti-Aldolase (Rabbit Muscle) was used at a 1:1000 dilution to detect human aldolase by Western blot. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.

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IHC‑plus™ Polyclonal Goat anti‑Rabbit ALDOA / Aldolase A Antibody (Lyophilized, IHC, WB) LS‑B1461

Name: IHC-plus™ ALDOA / Aldolase A Polyclonal Antibody Goat Lyophilized | LSBio
Brand: Lifespan BioSciences
SKU: LS-B1461-500
Price: 485.0000 USD
Availability: InStock
Rating: 5 (1 reviews)

IHC Validated

(5.0) Rating

Submit Review (2) Publication(s) LSBio Guarantee

Note: This antibody replaces LS-C19090, LS-C34938, LS-C34937, LS-C142423

Antibody:

ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Rabbit, Human

Format:

Unconjugated, Lyophilized

$485

LS-B1461-500

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) Antibody

Application:

IHC, IHC-P, WB, IP, ELISA

Reactivity:

Rabbit, Human

Format:

Unconjugated, Lyophilized

Specifications

Description

Aldolase A antibody LS-B1461 is an unconjugated goat polyclonal antibody to Aldolase A (ALDOA) from rabbit. It is reactive with human and rabbit. Validated for ELISA, IHC, IP and WB. Cited in 2 publications.

(2) Publication(s)

Target

Rabbit ALDOA / Aldolase A

Synonyms

See All ALDOA / Aldolase A Antibodies

Host

Goat

Reactivity

Rabbit, Human (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Delipidation, salt fractionation and ion exchange chromatography

Modifications

Lyophilized

Immunogen

Aldolase [Rabbit Muscle].

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, purified and partially purified Aldolase [Rabbit Muscle].

Applications

IHC
IHC - Paraffin (5 µg/ml)
Western blot (1:1000 - 1:5000)
Immunoprecipitation (1:100)
ELISA (1:7000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Immunohistochemistry: LS-B1461 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1461 was determined to be 5 ug/ml.

Presentation

Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitution

Reconstitute in 100 µL of deionized water.

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About ALDOA / Aldolase A

Uniprot: P04075 NCBI: NM_000034 NP_000025.1

Validation

Anti-Aldolase antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Anti-Aldolase antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

See More About...

About LSBio Validation process

LSBio Ratings

IHC-plus™ ALDOA / Aldolase A Antibody (Lyophilized) for IHC, WB/Western, IP, ELISA LS-B1461 has an LSBio Rating of

(5.0) Rating

Publications (4.1)

Laboratory Validation Score (4)

Learn more about The LSBio Ratings Algorithm

Publications (2)

Proteomic profiling of osteosarcoma cells identifies ALDOA and SULT1A3 as negative survival markers of human osteosarcoma. Chen X, Yang TT, Zhou Y, Wang W, Qiu XC, Gao J, Li CX, Long H, Ma BA, Ma Q, Zhang XZ, Yang LJ, Fan QY. Molecular carcinogenesis. 2014 Feb;53:138-44.

Full Text Article PubMed: 22949271

The deubiquitinase JOSD2 is a positive regulator of glucose metabolism. Lyudmila Krassikova , Boxi Zhang , Divya Nagarajan, André Lima Queiroz, Merve Kacal, Evangelos Samakidis, Helin Vakifahmetoglu-Norberg, Erik Norberg. Cell death and differentiation. 2020 October;

Full Text Article PubMed: 33082514 PMC: PMC7937685

Submit a Publication for LS-B1461