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Anti-ACTB / Beta Actin Antibody (C-Terminus) IHC-plus™ LS-B1040

Note: This antibody replaces LS-C19587

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

ACTB / Beta Actin Antibody LS-B1040 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Beta Actin antibody IHC of human skin.
Immunohistochemistry
Anti-Beta Actin antibody IHC of human prostate.
Immunocytochemistry
Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10ug/ml.
Immunofluorescence
Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 20 ug/ml.
Western blot
Western blot of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and...

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Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human ACTB / Beta Actin
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), ICC, Western blot

Specificity and Use

ACTB / Beta Actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human b-actin.
LS-E30227 - Liquid - 50 µg - $145.00
C-Terminus
A sixteen amino acid peptide near the C-terminus of human beta actin
Immunohistochemistry: LS-B1040 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1040 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About ACTB / Beta Actin

P60709 NM_001101 NP_001092.1

ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody

Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.

Immunohistochemistry

Immunohistochemistry
Anti-Beta Actin antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1040 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-Beta Actin antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1040 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 20 ug/ml.

Western blot

Western blot
Western blot of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
 

Requested From: United States
Date Requested: 8/3/2015

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