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Catalog Number Size Price
LS-B1625-50 50 µl (0.15 mg/ml) $505 
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
ACTB / Beta Actin Antibody - Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
ACTB / Beta Actin Antibody - Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
ACTB / Beta Actin Antibody - Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
ACTB / Beta Actin Antibody - Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.
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IHC‑plus™ Polyclonal Rabbit anti‑Mouse ACTB / Beta Actin Antibody (N‑Terminus, IHC, WB) LS‑B1625

IHC‑plus™ Polyclonal Rabbit anti‑Mouse ACTB / Beta Actin Antibody (N‑Terminus, IHC, WB) LS‑B1625

Note: This antibody replaces LS-C41293
Antibody:
ACTB / Beta Actin Rabbit anti-Mouse Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$505
LS-B1625-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Mouse Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-B1625 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from mouse. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB. Cited in 17 publications.
Target
Mouse ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from mouse ACTB / Beta-actin.
Epitope
N-Terminus
Specificity
Peptide-KLH, NH2 terminus
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1625 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1625 was determined to be 1:100.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

Validation

ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Beta Actin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

IHC-plus™ ACTB / Beta Actin Antibody (N-Terminus) for IHC, ICC, WB/Western LS-B1625 has an LSBio Rating of
Publications (5.6)
Laboratory Validation Score (4)

Publications (17)

Gap-junctional communication is required for mitotic clonal expansion during adipogenesis. Yanagiya T, Tanabe A, Hotta K. Obesity (Silver Spring, Md.). 2007 15:572-82.

IL-21 induces inhibitor of differentiation 2 and leads to complete abrogation of anaphylaxis in mice. Kishida T, Hiromura Y, Shin-Ya M, Asada H, Kuriyama H, Sugai M, Shimizu A, Yokota Y, Hama T, Imanishi J, Hisa Y, Mazda O. Journal of immunology (Baltimore, Md. : 1950). 2007 179:8554-61.

Epoxycholesterol impairs cholesteryl ester hydrolysis in macrophage foam cells, resulting in decreased cholesterol efflux. Ouimet M, Wang MD, Cadotte N, Ho K, Marcel YL. Arteriosclerosis, thrombosis, and vascular biology. 2008 28:1144-50. (WB)

Activated protein C up-regulates IL-10 and inhibits tissue factor in blood monocytes. Toltl LJ, Beaudin S, Liaw PC. Journal of immunology (Baltimore, Md. : 1950). 2008 181:2165-73.

Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. Sawada T, Nishiyama C, Kishi T, Sasazuki T, Komazawa-Sakon S, Xue X, Piao JH, Ogata H, Nakayama J, Taki T, Hayashi Y, Watanabe M, Yagita H, Okumura K, Nakano H. The Journal of biological chemistry. 2008 283:26820-8.

Knockdown of macrophage migration inhibitory factor disrupts adipogenesis in 3T3-L1 cells. Ikeda D, Sakaue S, Kamigaki M, Ohira H, Itoh N, Ohtsuka Y, Tsujino I, Nishimura M. Endocrinology. 2008 149:6037-42.

Muramyldipeptide augments the actions of lipopolysaccharide in mice by stimulating macrophages to produce pro-IL-1 and by down-regulation of the suppressor of cytokine signaling 1 (SOCS1). Shikama Y, Kuroishi T, Nagai Y, Iwakura Y, Shimauchi H, Takada H, Sugawara S, Endo Y. Innate immunity. 2011 17:15-Mar.

The pathogen recognition receptor NOD2 regulates human FOXP3+ T cell survival. Rahman MK, Midtling EH, Svingen PA, Xiong Y, Bell MP, Tung J, Smyrk T, Egan LJ, Faubion WA. Journal of immunology (Baltimore, Md. : 1950). 2010 184:7247-56.

Genetic deletion of the P2Y2 receptor offers significant resistance to development of lithium-induced polyuria accompanied by alterations in PGE2 signaling. Zhang Y, Pop IL, Carlson NG, Kishore BK. American journal of physiology. Renal physiology. 2012 302:F70-7.

c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis. Piao X, Komazawa-Sakon S, Nishina T, Koike M, Piao JH, Ehlken H, Kurihara H, Hara M, Van Rooijen N, Schtz G, Ohmuraya M, Uchiyama Y, Yagita H, Okumura K, He YW, Nakano H. Science signaling. 2012 5:ra93.

Predictive value of platelet aggregation rate in postpartum deep venous thrombosis and its possible mechanism. Sun M, Liu C, Zhao N, Meng K, Zhang Z. Experimental and therapeutic medicine. 2018 June;15:5215-5220. (Rabbit, Mouse)

URG11 promotes proliferation and induced apoptosis of LNCaP cells. Sun C, Zhang G, Cheng S, Qian H, Li D, Liu M. International journal of molecular medicine. 2019 May;43:2075-2085.

Knockdown of miR-222 inhibits inflammation and the apoptosis of LPS-stimulated human intervertebral disc nucleus pulposus cells. Zhang Y, Yang J, Zhou X, Wang N, Li Z, Zhou Y, Feng J, Shen D, Zhao W. International journal of molecular medicine. 2019 October;44:1357-1365.

Knockdown of KLK12 inhibits viability and induces apoptosis in human colorectal cancer HT-29 cell line. Li Q, Zhou X, Fang Z, Zhou H. International journal of molecular medicine. 2019 November;44:1667-1676.

miR-217 inhibits the migration and invasion of HeLa cells through modulating MAPK1. Zhu L, Yang S, Wang J. International journal of molecular medicine. 2019 November;44:1824-1832.

Effect of estradiol on high glucose-induced osteoblast injury. Li G, Jiang X, Liu L, Liu X, Liu H, Zhang Z. Molecular medicine reports. 2019 October;20:3019-3026.

GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Canjun Zhao #, Jin Zhang #, Litian Ma #, Hao Wu, Hui Zhang, Jialin Su, Bizu Geng, Qinghua Yao, Jin Zheng. OncoTargets and therapy. 2020 July;13:6265-6277.

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Date Requested: 1/21/2022