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Catalog Number Size Price
LS-B323-50 50 µg (0.9 mg/ml) $440 
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ACTB / Beta Actin Antibody - Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
ACTB / Beta Actin Antibody - Anti-Beta Actin Antibody. Tissue: sections 4.2 mm thick of rana pipiens tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Beta Actin antibody at 1:200 for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Beta Actin is at the neuromuscular junction. Staining: Beta Actin as precipitated green signal with red and blue counterstain.
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IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa350‑375, IHC, IF, WB) LS‑B323

IHC‑plus™ Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (aa350‑375, IHC, IF, WB) LS‑B323

Note: This antibody replaces LS-C19049, LS-C56854
Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa350-375) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B323-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (aa350-375) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-B323 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (aa350-375) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Epitope
aa350-375
Specificity
Reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:1000 - 1:4000)
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B323 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B323 was determined to be 5 ug/ml.Antibody has been tested in IHC with human and frog samples and in Western blot with human and mouse samples. It is expected to react with a wide range of species due to sequence homology.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

Validation

ACTB / Beta Actin Antibody - Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Beta Actin antibody IHC of human skeletal muscle, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ ACTB / Beta Actin Antibody (aa350-375) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B323 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/23/2022