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Anti-ACTB / Beta Actin Antibody (aa359-368, Biotin) LS-C154297

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACTB / Beta Actin Antibodies

Anti-ACTB / Beta Actin Antibody (C-Terminus) IHC-plus™ LS-B1040
Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACTB / Beta Actin Antibody (N-Terminus, HRP) LS-C126575
Rabbit Monoclonal (IgG) to Human ACTB / Beta Actin
Human, Monkey, Mouse, Rat, Bovine, Pig
Western blot
HRP Conjugated
Anti-ACTB / Beta Actin Antibody (aa2-16) IHC-plus™ LS-B6503
Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human ACTB / Beta Actin
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep
IHC, Western blot, ELISA
Biotin Conjugated

Details

Human ACTB / Beta Actin
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Goat, Guinea pig, Hamster, Pig, Sheep (tested or 100% immunogen sequence identity)
IgG Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA

Specificity and Use

ACTB / Beta Actin antibody was raised against this affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.
aa359-368
A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.
This affinity-purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kD in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About ACTB / Beta Actin

P60709 NM_001101 NP_001092.1

ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody

Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.

Requested From: United States
Date Requested: 12/3/2016

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