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Anti-ACTB / Beta Actin Antibody (aa2-16) IHC-plus™ LS-B6503

Note: This antibody replaces LS-C19378

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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ACTB / Beta Actin Antibody LS-B6503 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Beta Actin antibody IHC of human spleen.
Western blot
Western blot of beta actin in A: human brain (40141), B: mouse brain (40121), C: rat brain...
Western blot
Western blot of beta actin in decreasing amounts of human ovary tissue lysate using antibody at...

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Rabbit Polyclonal to Human ACTB / Beta Actin
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated

Details

Human ACTB / Beta Actin
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Monkey, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus, Insect (at least 90% immunogen sequence identity)
Polyclonal
Protein G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (0.25 - 1 µg/ml)

Specificity and Use

ACTB / Beta Actin antibody was raised against amino acids 2-15 (CDDDIAALVIDNGSG) of human beta actin were used as the immunogen. Percent identity by BLAST analysis: Seq squirt, Sea anemone (100%); Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Guinea pig, Chicken, Platypus, Xenopus, Medaka, Mosquito, Ant (93%).
aa2-16
Amino acids 2-16 (CDDDIAALVIDNGSG) of actin protein were used as the immunogen.

Packaging

PBS containing 0.05% BSA and 0.05% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About ACTB / Beta Actin

P60709 NM_001101 NP_001092.1

ACTB Antibody, Actin, cytoplasmic 1 Antibody, Beta-actin Antibody, Beta actin Antibody, BRWS1 Antibody, Actin, beta Antibody, Beta cytoskeletal actin Antibody, PS1TP5-binding protein 1 Antibody, PS1TP5BP1 Antibody

Actin is a highly conserved protein that participates in a wide variety of cellular functions in eukaryotes including muscle contraction, amoeboid movement, cytokinesis and mitotic division. Beta actin is ubiquitously expressed in all eukaryotic cells.

Immunohistochemistry

Immunohistochemistry
Anti-Beta Actin antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6503 concentration 10 ug/ml.

Western blot

Western blot
Western blot of beta actin in A: human brain (40141), B: mouse brain (40121), C: rat brain (40121), D: human lung (40146) and E: human spleen (40149)tissue lysates using antibody at 0.25 ug/ml.

Western blot

Western blot
Western blot of beta actin in decreasing amounts of human ovary tissue lysate using antibody at 0.25 ug/ml: A) 40 ug, B) 30 ug, C) 20 ug, and D) 10 ug per lane.
 

Requested From: United States
Date Requested: 8/3/2015

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