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Catalog Number Size Price
LS-C209628-100 100 µg (1 mg/ml) $552 
ALDOA / Aldolase A Antibody - Anti aldolase antibody – Immunoprecipitation and Western Blot. 300 µl aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and Western blot. Samples shown in the image are: 1. Purified aldolase 2. 300 µl antiserum with no antigen (negative control) 3. 300 µl antiserum with ~100 µl aldolase (2.5 mg/ml) 4. 300 µl antiserum with ~200 µl aldolase (2.5 mg/ml) For the precipitation, 300 ul of antiserum and an equal volume of aldolase antigen in PBS was incubated ~24 hrs at 4°C, centrifuged for 6 minutes at 13K RPM, washed once with PBS, centrifuged and dissolved in 60 ul 0.1 N NaOH.  90 ul of PBS was added, the sample was divided in 2 portions, and an equal volume of reducing (+4% BME) or non-reducing 2X sample buffer was added.  The reduced samples were boiled for five minutes, and all samples were run at 140 V for ~45 minutes on a 4-20% tris/glycine gradient gel.

Polyclonal Goat anti‑Rabbit ALDOA / Aldolase A Antibody (Lyophilized, Biotin, WB) LS‑C209628

Polyclonal Goat anti‑Rabbit ALDOA / Aldolase A Antibody (Lyophilized, Biotin, WB) LS‑C209628

Antibody:
ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Rabbit, Human
Format:
Biotin, Lyophilized
Price
Catalog Number
$552
LS-C209628-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ALDOA / Aldolase A Goat anti-Rabbit Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Rabbit, Human
Format:
Biotin, Lyophilized

Specifications

Description
Aldolase A antibody LS-C209628 is a biotin-conjugated goat polyclonal antibody to Aldolase A (ALDOA) from rabbit. It is reactive with human and rabbit. Validated for ELISA, IP and WB.
Target
Rabbit ALDOA / Aldolase A
Synonyms
ALDOA | ALDA | Aldolase | GSD12 | Muscle-type aldolase | Lung cancer antigen NY-LU-1
Host
Goat
Reactivity
Rabbit, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Aldolase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle]. Cross reactivity against Aldolase from other sources may occur but have not been specifically determined.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:4000)
Usage
This product has been assayed against 1.0 ug of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:16000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALDOA / Aldolase A
P04075 NM_000034 NP_000025.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/28/2024