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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGAP2 / PIKE Antibody (aa1-836) IHC-plus™ LS-B338

Note: This antibody replaces LS-C18961
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
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AGAP2 / PIKE Antibody (aa1-836) IHC-plus™ LS-B338

Note: This antibody replaces LS-C18961
Antibody:
Rabbit Polyclonal to Human AGAP2 / PIKE
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B338-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human AGAP2 / PIKE
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PIKE antibody LS-B338 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human AGAP2 / PIKE
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:5000 - 1:20000)
  • ELISA (1:40000 - 1:200000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AGAP2 / PIKE antibody was raised against recombinant human AGAP2 / PIKE.
Epitope
aa1-836
Specificity
Amino acids 1-836 of human AGAP2 protein.
Usage
Immunohistochemistry: LS-B338 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B338 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AGAP2 / PIKE
Q99490 NM_014770 NP_055585.1

Validation

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Reactivity:
Range: 12.5-200 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Rat
Range: 7.8-500 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Anti-ELANE / Neutrophil Elastase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Immunoelectrophoresis

Requested From: United States
Date Requested: 7/16/2019
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