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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AGAP2 / PIKE Antibody (aa1‑836) IHC‑plus™ LS‑B338
Note: This antibody replaces LS-C18961
PIKE antibody LS-B338 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B338-50
50 µg (1 mg/ml)
$425

Popular AGAP2 / PIKE Products

Anti-AGAP2 / PIKE antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B134 concentration 20 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
CENTG1 Antibody western blot of mouse brain tissue lysates (35 ug/lane). The CENTG1 antibody detected the CENTG1 protein (arrow).
Species: Human, Mouse
Applications: Western blot
Anti-AGAP2 / PIKE antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4387 concentration 3.75 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

PIKE antibody LS-B338 is an unconjugated rabbit polyclonal antibody to PIKE (AGAP2) from human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About AGAP2 / PIKE
Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity. PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin gamma 1. Q99490 NM_014770 NP_055585.1

AGAP2 Antibody, AGAP-2 Antibody, Centaurin, gamma 1 Antibody, Cnt-g1 Antibody, Centaurin-gamma-1 Antibody, KIAA0167 Antibody, GGAP2 Antibody, PIKE Antibody, CENTG1 Antibody


Specifications

Target
Human AGAP2 / PIKE
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1:5000 - 1:20000)
  • ELISA (1:40000 - 1:200000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AGAP2 / PIKE antibody was raised against recombinant human AGAP2 / PIKE.
Epitope
aa1-836
Specificity
Amino acids 1-836 of human AGAP2 protein.
Usage
Immunohistochemistry: LS-B338 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B338 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.2, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.

Western blot

Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.

Western blot

Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.

Western blot

Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.

Immunohistochemistry

Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.
Anti-AGAP2 / PIKE antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B338 concentration 5 ug/ml.

Western blot

Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.
Anti-AGAP2 Antibody - Western Blot. Western blot of Protein A purified anti-AGAP2 antibody shows detection of AGAP2 recombinant protein in transfected HEK293 cell lysates. No specific band staining is noted in mock transfected cell lysates (empty vector). The membrane was probed with the primary antibody diluted to 1:10000. Personal Communication, Paul Randazzo, CCR-NCI, Bethesda, MD.

Requested From: United States
Date Requested: 9/23/2018

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