Beta Actin antibody LS-C20277 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Synthetic peptide from the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.
Recognizes human beta Actin. Species cross-reactivity: mouse, rat, monkey. Expected to cross-react with a wide range of species due to sequence homology.
Western blot (1:500 - 1:2500)
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control. Immunofluorescence: 1:50. Positive control: HeLa whole cell lysate and 3T3 cells (mouse).