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Catalog Number Size Price
LS-C20277-50 50 µl $602 
Antibody

Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (N‑Terminus, IF, WB) LS‑C20277

Polyclonal Rabbit anti‑Human ACTB / Beta Actin Antibody (N‑Terminus, IF, WB) LS‑C20277

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$602
LS-C20277-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human, Monkey, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-C20277 is an unconjugated rabbit polyclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IP and WB.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-terminus of human beta Actin. Cellular Localization: Cytoplasmic.
Epitope
N-Terminus
Specificity
Recognizes human beta Actin. Species cross-reactivity: mouse, rat, monkey. Expected to cross-react with a wide range of species due to sequence homology.
Applications
  • ICC
  • Immunofluorescence (1:50)
  • Western blot (1:500 - 1:2500)
  • Immunoprecipitation
Usage
Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500-1:2500. Can be blocked with beta Actin peptide (aa2-15). At higher concentrations, other bands can be detected. Note: May be used as a cell extract loading control. Immunofluorescence: 1:50. Positive control: HeLa whole cell lysate and 3T3 cells (mouse).
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/11/2022