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Catalog Number Size Price
LS-C153787-100 100 µg (1 mg/ml) $574 
ACTB / Beta Actin Antibody - Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N-Term antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 3H2.E4.E12, N‑Terminus, IHC, WB) LS‑C153787

Monoclonal Mouse anti‑Human ACTB / Beta Actin Antibody (clone 3H2.E4.E12, N‑Terminus, IHC, WB) LS‑C153787

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (N-Terminus) (3H2.E4.E12) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C153787-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ACTB / Beta Actin Mouse anti-Human Monoclonal (N-Terminus) (3H2.E4.E12) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human, Mouse, Rat, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Beta Actin antibody LS-C153787 is an unconjugated mouse monoclonal antibody to Beta Actin (ACTB) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human ACTB / Beta Actin
Synonyms
ACTB | Actin, cytoplasmic 1 | Beta-actin | Beta actin | BRWS1 | Actin, beta | Beta cytoskeletal actin | PS1TP5-binding protein 1 | PS1TP5BP1
Host
Mouse
Reactivity
Human, Mouse, Rat, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG2a,l Monoclonal
Clone
3H2.E4.E12
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.
Epitope
N-Terminus
Specificity
A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates. Cross-reactivity with other sources has not been determined.
Applications
  • IHC
  • Western blot (1 µg/ml)
  • ELISA (1:5000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-beta Actin antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTB / Beta Actin
P60709 NM_001101 NP_001092.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024