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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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WNT1

wingless-type MMTV integration site family, member 1

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region.

Gene Name: wingless-type MMTV integration site family, member 1
Family/Subfamily: WNT , not assigned-WNT
Synonyms: WNT1, INT1, Proto-oncogene Int-1 homolog, Proto-oncogene Wnt-1
Target Sequences: NM_005430 NP_005421.1 P04628

Filtered by: None
Target
WNT1 (81)
Reactivity
Human (81)
Mouse (42)
Rat (27)
Bat (12)
Bovine (25)
Chimpanzee (6)
Dog (17)
Gibbon (6)
Guinea pig (9)
Hamster (12)
Horse (23)
Macaque (1)
Monkey (16)
Opossum (1)
Pig (24)
Rabbit (23)
Zebrafish (4)
Application
IHC (45)
IHC-P (20)
WB (61)
Flo (3)
ELISA (21)
ICC (2)
IF (7)
IP (7)
Peptide-ELISA (1)
Host
rabbit (77)
mouse (4)
Isotype
IgG (28)
IgG1 (3)
Clonality
monoclonal mc (4)
polyclonal pc (77)
Clone
10C8 (2)
13F9 (1)
Format
AP Conjugated (5)
APC Conjugated (5)
Atto 390 Conjugated (1)
Atto 488 Conjugated (1)
Atto 565 Conjugated (1)
Atto 594 Conjugated (2)
Atto 633 Conjugated (1)
Atto 655 Conjugated (1)
Atto 680 Conjugated (1)
Atto 700 Conjugated (1)
Azide-free (2)
Biotin Conjugated (9)
FITC Conjugated (8)
HRP Conjugated (8)
PE (1)
PE Conjugated (4)
PerCP Conjugated (1)
RPE Conjugated (1)
SA Conjugated (1)
Unconjugated (30)
Epitope
Internal (16)
aa180-229 (4)
aa297-346 (4)
C-Terminus (2)
270-350 aa, C-terminal (1)
Gly331 (1)
aa260-288 (1)
aa301-350 (1)
aa45-74 (1)
Publications
No (81)
Showing 1-25 of 81 results

IHC-plus™ WNT1 Antibody (Internal) LS-A9611
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, Biotin) LS-A109681
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, FITC) LS-A109682
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
FITC Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, AP) LS-A109679
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
AP Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, APC) LS-A109680
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, PE) LS-A109684
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
PE Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, Azide-free, HRP) LS-A109683
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Dog, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P
HRP Conjugated, Azide-free
100µl  $650.00
Anti-WNT1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (clone 10C8) LS-B4117
Mouse Monoclonal [clone 10C8] (IgG1) to Human WNT1
Human
ELISA, Flo, IF, IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Anti-WNT1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Figure3: Confocal immunofluorescence of HeLa (left) and 3T3-L1 (right) cells using WNT1 mouse monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

WNT1 Antibody (C-Terminus) LS-C353005
Rabbit Polyclonal to Human WNT1
Mouse, Bovine, Rat, Pig, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of WNT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of WNT1 staining in H9C2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

WNT1 Antibody (aa260-288) LS-C160627
Rabbit Polyclonal to Human WNT1
Mouse, Human
Flo, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
WNT1 Antibody IHC of formalin-fixed and paraffin-embedded human testis followed by peroxidase-conjugated secondary antibody and DAB staining.
Western blot of WNT1 Antibody in mouse heart tissue lysates (35 ug/lane). WNT1 (arrow) was detected using the purified antibody.

IHC-plus™ WNT1 Antibody LS-B7478
Rabbit Polyclonal (IgG) to Human WNT1
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg  $425.00
Anti-WNT1 antibody IHC of human brain, cerebellum, Purkinje. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

WNT1 Antibody (clone 10C8) LS-C758792
Mouse Monoclonal [clone 10C8] (IgG1) to Human WNT1
Mouse, Human
Flo, ICC, IF, IHC, WB
Unconjugated
100µl  $445.00
WNT1 Antibody in Immunohistochemistry (IHC)
WNT1 Antibody in Immunofluorescence (IF)

WNT1 Antibody (Biotin) LS-C104707
Rabbit Polyclonal to Human WNT1
Human
ELISA, WB
Biotin Conjugated
25µg  $285.00; 50µg  $325.00

WNT1 Antibody LS-C801420
Rabbit Polyclonal (IgG) to Human WNT1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

WNT1 Antibody (270-350 aa, C-terminal) LS-C386673
Rabbit Polyclonal (IgG) to Human WNT1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Wnt-1 antibody
Western blot of Wnt-1 antibody

WNT1 Antibody (Gly331) LS-C176622
Rabbit Polyclonal to Human WNT1
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of WNT1 (G331) pAb in paraffin-embedded human breast carcinoma tissue.
Western blot of Wnt-1 (G331) pAb in extracts from HeLa cells.

IHC-plus™ WNT1 Antibody (Internal) LS-A8568
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, Biotin) LS-A108067
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
Biotin Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, FITC) LS-A108068
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
FITC Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, AP) LS-A108065
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
AP Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, APC) LS-A108066
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
APC Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, PE) LS-A108070
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
PE Conjugated
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ WNT1 Antibody (Internal, Azide-free, HRP) LS-A108069
Rabbit Polyclonal to Human WNT1
Rabbit, Mouse, Bovine, Rat, Pig, Horse, Human
IHC, IHC-P
HRP Conjugated, Azide-free
100µl  $650.00
Anti-WNT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-WNT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

WNT1 Antibody LS-C417134
Rabbit Polyclonal to Human WNT1
Mouse, Human
IF, IHC, WB
Unconjugated
100µg  $305.00

WNT1 Antibody LS-C406137
Rabbit Polyclonal (IgG) to Human WNT1
Mouse, Human
ELISA, IHC, WB
Unconjugated
20µl 0.4mg/ml  $205.00; 60µl 0.4mg/ml  $235.00; 120µl 0.4mg/ml  $295.00; 200µl 0.4mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human thyroid cancer using WNT1 Polyclonal Antibody at dilution of 1:50.
Western blot analysis of HT-29 cell, using WNT1 Polyclonal Antibody at dilution of 1:300.
Showing 1-25 of 81 results

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PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).